To establish a model with a short modeling period and consistent with the pathogenesis of IBS, chronic stimulation and chemical stimulation were combined. In the present study, we aimed to establish a novel model of irritable bowel syndrome (IBS) and investigate its feasibility. Chronic unpredictable stimulation was used as a single factor model, and its addition with glacial acetic acid stimulation was used as a comprehensive factor model. The symptoms of the IBS model were analyzed from a collection of tests, including body weight, essential food consumption, sucrose consumption, fecal grain number, Bristol score, abdominal withdrawal reflex, open field tests, elevated cross-maze tests, intestinal transportation time, and H&E staining. The results showed that compared with the single-factor model and the control groups, the rats in the comprehensive model group exhibited less body weight, reduced food consumption, decreased sucrose consumption, increased fecal grain number, decreased Bristol score and pain threshold, and significantly reduced physical activity. The comprehensive factor group model conformed to the complex pathogenesis of IBS and thus had a significant effect on it, which could be used to establish an effective IBS model.?Furthermore, the modeling effect was significantly evident in male rats compared with female ones.