Table of Content

    30 June 2020, Volume 29 Issue 6
    Pharmacist-led interventions to reduce inappropriate drug use in the orthopaedics department in China
    Chengxin Xie, Wei Wang, Ge Huang, Xin Jin, Chenglong Wang, Dong Yin
    2020, 29(6):  375-382.  DOI: 10.5246/jcps.2020.06.035
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    As the demand for pharmaceutical services continues to grow, more and more attention has been paid to the pharmaceuticalcare effect. However, in China, the training pathways and practical work of clinical pharmacy are still in the exploratory stage. In the present review, we aimed to discuss the impact of pharmacist-led interventions in reducing inappropriate drug use in orthopaedics.Results showed clinical pharmacists can assist orthopaedists and nurses in the rational use of medication in all aspects of antibiotictherapy, pain control and anticoagulation therapy. The clinical interventions by pharmacists have greatly reduced medication errors and medical costs. However, there is still a long time to develop pharmacy service, improve the professional quality of pharmacists and enact the Chinese Pharmacist Law.
    Original articles
    Virtual screening for triple-negative breast cancer cell inhibitors based on telomere G-quadruplex structure
    Hua Deng, Chao Gao, Dengguo Wei, Sisi Liu
    2020, 29(6):  383-389.  DOI: 10.5246/jcps.2020.06.036
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    Triple-negative breast cancer is an aggressive subtype that frequently develops resistance to chemotherapy. It is expectedto develop new anti-tumor drugs through targeting the structure of G-quadruplexes of the genes associated with this tumor. In this work, by targeting the 21-mer telomere G-quadruplex structure, compounds VB07 and VC02 were identified to stabilize the telomere G-quadruplex through structure-based high-throughput virtual screening. Cell cytotoxicity assay showed that VB07 and VC02 exhibited inhibitory effect on triple-negative breast cancer cells at the concentration of 5 μM. This study showed that structure-based high-throughput virtual screening was able to successfully identify the proper compounds targeting the telomere G-quadruplex, which exhibited inhibitory effects against thetriple-negative breast cancer cells. 
    Insights from investigating the interactions of natural product inhibitors with neuraminidase of the 2009 H1N1 influenza virus
    Yurui Jin, Aixiu Li, Jiaxiong Kang
    2020, 29(6):  390-397.  DOI: 10.5246/jcps.2020.06.037
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    Neuraminidase (NA) plays a biologically vital role in the replication of influenza virus, and NA inhibitors (NAIs) are most widely used in the clinical anti-flu therapy. NA of 2009 H1N1 influenza virus (09N1) possesses a different substrate-binding cavity compared with other NA subtypes, making 09N1 a more appropriate starting point for the discovery of potent 09N1 inhibitors. As natural products are of great structural diversity, research on the interaction between natural NAIs and 09N1 can throw light on the design of new structural NAIs. In this study, we, for the first time, conducted molecular docking procedure with GOLD on 10 natural inhibitors to 09N1, and acquired their binding modes with 09N1. The docking results showed that the active site S1 was important in the binding of NAIs to 09N1. Then five scaffolds were extracted from these NAIs with interactions to site S1, and these could be used in the structural modification of NAIs. Besides, we found that the addition of H-bonding interaction with the active site could improve the NA inhibitory activity of NAIs, and it might be the reason why the approved NAIs showed high efficiency. Two terminal hydrophobic sites (Terminal 1 and Terminal 2) with no interactions to the approved NAI zanamivir were found in the 09N1 active cavity, and four NAIs were first found to bind with the terminals. Till now, there are few studies on the meaning of Terminal 2 in the binding of NAI to NA, which could be a new direction for the rational design of NAIs.
    Curcumin inhibits cervical cancer cell proliferation, migration and invasion by suppressing the PI3K/AKT signaling pathway via the miR-29b/KDM2A axis
    Xichun Guo, Jinghua Chen, Rujun Gao, Xiuyuan Han
    2020, 29(6):  398-410.  DOI: 10.5246/jcps.2020.06.038
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    In this study, we aimed to examine whether curcumin exerted its anti-tumor effects by regulating miR-29b/KDM2A in cervical cancer cells. The cell viability, migration and invasion were estimated in HeLa cervical cancer cells treated with curcumin. The effects of microRNA-29b (miR-29b) on biological behaviors of HeLa SiHa cells were also assessed. Potential target genes of miR-29b were predicted and confirmed using a luciferase reporter assay, and the effects of curcumin and miR-29b on the PI3K/AKTsignaling pathway were analyzed. Curcumin treatment inhibited cell proliferation, migration and invasion of HeLa cells (P<0.05). The miR-29b expression was promoted by curcumin treatment in HeLa cells (P<0.01), and miR-29b depletion could restore the effects of curcumin on cell proliferation, migration and invasion of HeLa cells (P<0.05). KDM2A was proved as a direct target gene of miR-29b, and the activity of the PI3K/AKT signaling could be regulated by curcumin and miR-29b (P<0.05). All the data revealed that curcumin played a protective role in cervical cancer. The proliferation, migration and invasion of cervical cancer cells were inhibited by curcumin through the miR-29b/KDM2A/PI3K/AKT pathway.
    Determination of free fatty acids in rabbit plasma by GC-MS after trimethylsilylation derivatization
    Jialin Du, Liangjiu Yang, Kezhao Wei, Can Gong, Jianping Gao, Xu Xu
    2020, 29(6):  411-421.  DOI: 10.5246/jcps.2020.06.039
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    In the present study, free fatty acids (FFAs, including palmitic acid, stearic acid, oleic acid, linoleic acid and arachidonicacid) in rabbit plasma were determined by gas chromatography-mass spectrometry (GC-MS) after trimethylsilylation derivatizationusing N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) – trimethylchlorosilane (TMCS) as derivatization reagent. The experimental conditions, including extraction and silylation reaction, were investigated. The method was experimentally validated. The linearity between fatty acids’ peak areas and their concentrations was obtained with the corelative coefficient (r2) all more than 0.999, and the recoveries were between 82% and 111%. The intra-day variations of FFAs’ in plasma samples at different concentrations were all less than 6%. FFA analysis results of 16 rabbit plasma samples showed that the method could be well applied in the determination of plasma samples in vivo. In contrast to the traditional method of FFA derivatization, the established trimethylsilylationmethod presented simplicity, high specificity, and completely free from the interference of the esterified fatty acid, such as triacylglyceride. The method could be applied for analyzing FFA profiles in the clinical laboratory or pharmacological research. 
    Application of a quantitative 1H NMR method for rapid extraction and determination of the content of paeonol in Cynanchum paniculatum
    Xuejiao Li, Jianwei Dong, Xiu Gao, Guijun Li, Junyou Shi, Yanqing Zhang
    2020, 29(6):  422-430.  DOI: 10.5246/jcps.2020.06.040
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    Cynanchum paniculatum (Bunge) Kitagawa is usually used as an herbal medicine for treating many diseases. Paeonol is the main active component, and its content is the key indicator for quality control of C. paniculatum. In the present study, we developed a rapid, accurate and precise method for quantitation of paeonol in C. paniculatum using 1H NMR spectra. The deuteratedsolvent of methanol-d4 enabled satisfactory separation of the signals to be integrated in 1H NMR spectrum. H-6 (δ 7.78) of1H NMR spectrum of C. paniculatum was selected as the feature signal for quantitation, and trimesic acid (TMA) was selected as an internal standard. Validation of the quantitative method was performed in terms of linearity, specificity, repeatability and stability. This is the first time to report quantitative 1H NMR(qHNMR) applied to determine the content of paeonol in C. paniculatum and showed a wider linearity range than the reported quantitation of paeonol in others. The simple extraction of paeonol from C. paniculatum was rapid and will prompt the application of the developed method. This work implied that qHNMR represented a feasible alternative to HPLC-based methods for quantitation of paeonol in C. paniculatum, and it was suitable for the quality control of C. paniculatum. 
    Chemical constituents from Trichosanthes cucumeroides
    Jing Chen, Li Cheng, Fuqian Wang, Xuanbin Wang, Yonghui Zhang, Xincai Hao
    2020, 29(6):  431-438.  DOI: 10.5246/jcps.2020.06.041
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    One new triterpenoid, 3β-[(E)-caffeoyloxy]-D:C-friedooleana-7,9(11)-dien-29-oic acid (1) and nine known triterpenoidsidentified as cucurbitacin B (2), 23,24-dihydrocucurbitacin B (3), 23,24-dihydrocu-curbitacin D (4), cucurbitacin D (5), cucurbalsaminol A (6), 3-epi-isocucurbitacin D (7), curbitacin G (8), cucurbitacin J (9) and cucurbitacin I (10)were purified from Trichosanthes cucumeroides. Their structures were confirmed by spectroscopic data analysis and comparison with previous literature. All compounds were obtained from T.cucumeroides for the first time.  
    The others
    The group of Prof. Yanxing Jia have realized the first total synthesis of natural product (-)-glaucocalyxin A
    Shuxiang Song
    2020, 29(6):  439-440. 
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    The group of Prof. Yanxing Jia have realized the first total synthesis of natural product (-)-glaucocalyxin A.
    The group of Prof. Xianrong Qi have provided a new delivery strategy for accurate diagnosis and treatment of glioma
    School of Pharmaceutical Sciences, Peking University Health Science Center
    2020, 29(6):  441-442. 
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    The group of Prof. Xianrong Qi have provided a new delivery strategy for accurate diagnosis and treatment of glioma.