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    Development of oral pharmaceutical formulation of standardized crude ethanolic extract of Atractylodes lancea (Thunb) DC.
    Thananchanoke Rattanathada, Tullayakorn Plengsuriyakarn, Rathapon Asasujarit, Anurak Cheoymang, Juntra Karbwang, Kesara Na-Bangchang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (4): 280-293.   DOI: 10.5246/jcps.2020.04.027
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    Cholangiocarcinoma (CCA), the adenocarcinoma of the biliary duct, is commonly reported in Asia with the highest incidence in northeastern Thailand. Chemotherapy of this type of cancer is limited due to the lack of effective chemotherapeutic drugs . A series of previous studies support further research and development of Atractylodes lancea (Thunb) DC. (AL) as a potential candidate for the treatment of CCA as a crude ethanolic extract. In the present study, we aimed to develop an oral pharmaceutical formulation (capsule) of th e standardized AL crude ethanolic extract for further clinical development in patients with CCA. Major steps included macroscopic and microscopic authentication of the AL rhizomes, preparation of standardized AL extract, preparation of oral pharmaceutical formulation (capsule) of the standardized AL extract, quantitative and qualitative analysis of the marker compound (atractylodin) in the formulated AL extract, evaluation of contaminations of heavy metal s, pesticides residues, and microorganisms in the ground AL rhizomes and the formulated (capsule) powder of AL, physicochemical and pharmaceutical properties of the formulated AL extract/capsule, and cytotoxicity evaluation of the formulated AL extract. Results of all evaluations confirmed satisfactory pharmaceutical properties of oral (capsule) formulation of the standardized AL extract.
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    Plant pharmacophylogeny: past, present and future
    Dacheng Hao, Peigen Xiao
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 831-854.   DOI: 10.5246/jcps.2020.12.075
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    The concept of "pharmacophylogeny" was proposed by Peigen Xiao in the 1980s based on long-term studies of Chinese researchers since ancient times and especially the 1950s. The complicated relationships and connectivity between kinship of medicinal plants, their chemical profiles and therapeutic utilities are consistent goals of pharmacophylogeny studies, which benefit innovative drug R&D. In the present work, we reviewed the origin and a brief history of research in this field, as well as the status quo and recent progress of pharmacophylogeny. The concept "pharmacophylogenomics" is put forward to represent the expanding utility of pharmacophylogeny in botanical drug R&D. Pharmacophylogeny and pharmacophylogenomics are the synthesis of multiple disciplines, such as chemotaxonomy, plant morphology, plant biochemistry/molecular biology and omics, etc. Medicinal plants within the same phylogenetic groups may have the same or similar therapeutic compounds/effects, thus forming the core of pharmacophylogeny, which is the scientific law summed up from practice and applied to practice after refining and sublimation. In the past, pharmacophylogeny plays a big role in looking for alternative resources of imported drugs in China. At present, it continues to play an active role in expanding medicinal plant resources, quality control/identification of herbal medicines, as well as predicting the chemical constituents or active ingredients of herbal medicine and the identification and determination of chemical constituents. In the ongoing future, it will play a bigger role in the search for new drugs, sorting out, summarizing, and improving herbal medicine experiences, thus boosting the sustainable conservation and utilization of traditional/natural?medicinal resources.

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    Integrated combination delivery of IDO inhibitor and paclitaxel for cancer treatment
    Dakuan Wang, Bo Peng, Mengmeng Qin, Minghui Li, Ge Song, Bing He, Hua Zhang, Wenbing Dai, Qiang Zhang, Xiangbao Meng, Huan Meng, Xueqing Wang
    Journal of Chinese Pharmaceutical Sciences    2021, 30 (1): 1-16.   DOI: 10.5246/jcps.2021.01.001
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    In order to realize the combination of chemotherapy and immunotherapy, a reduction-responsive paclitaxel (PTX) prodrug PEG-SS-PTX was synthesized and used as a carrier to encapsulate IDO inhibitor CY-1-4 for preparing PEG-SS-PTX/CY-1-4 NPs. PEG-SS-PTX/CY-1-4 NPs were evaluated by cytotoxicity, immunogenic cell death (ICD) induction ability and anti-tumor efficacy. Dynamic light scattering (DLS) results showed that the size of PEG-SS-PTX/CY-1-4 NPs was about 149 nm. In vitro experiments indicated that its cytotoxicity was in a concentration-dependent manner, and it induced the ICD of B16-F10 cells. In vivo studies in melanoma mouse model indicated that PEG-SS-PTX/CY-1-4 NPs significantly inhibited the tumor growth and reduced the expression of IDO in tumor tissues. Moreover, it increased the rate of CD8+ T cells in the spleen. In summary, PEG-SS-PTX/CY-1-4 NPs achieved good anti-tumor effects and reduced the dose of chemotherapy drugs, which was a safe and effective combined delivery system.

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    DGMI alleviates OGD/R-induced cell injury by regulating inflammatory and apoptosis signaling pathways
    Qian Hong, Zenghui Wang, Yang Yang, Lu Gao, Zhao Yan
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (7): 455-469.   DOI: 10.5246/jcps.2020.07.043
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    Diterpene ginkgolides meglumine injection (DGMI), a kind of Ginkgo biloba special extract injection, is now used for the treatment of ischemic stroke in convalescence. In the present study, we aimed to confirm whether DGMI could suppress inflammatory responses and apoptosis and explore the potential mechanisms underlying these effects. Cell viability and lactate dehydrogenase (LDH) release were measured by MTS and LDH assays after the cells were exposed to oxygen-glucose deprivation/reoxygenation (OGD/R). The extent of anti-apoptotic effect of DGMI was detected by flow cytometry using Annexin V-FITC/PI double staining assay kit. Pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6 and IL-10, were quantified by a specific Bio-Plex Pro TM Reagent Kit. Additionally, activities of TLR2/4, NF-κB p65, MAPK pathway and apoptosis-related proteins as well as cellular localization of NF-κB p65 were determined by Western blotting analysis and immunofluorescence staining, respectively. DGMI at 50 μg/mL significantly increased the cell viability and decreased the secretion of IL-1β, IL-6, IL-10 and TNF-α in OGD/R-induced BV2 microglia cells. These effects were also confirmed by LDH assay and Annexin V-FITC/PI staining. Meanwhile, DGMI not only inhibited the protein expressions of TLR2, TLR4, MyD88, p-TAK1, p-IkBα, p-IKKβ and Bak, but also decreased the cleaved caspase-3/caspase-3, Bax/Bcl-2 and cleaved PARP-1/PARP-1 ratio in OGD/R-induced BV2 microglia cells. Furthermor e, OGD /R-enhanced p-JNK1/2 and p-p38 MAPK expressions and nuclear translocation of NF-κB p65 were also partially inhibited by DGMI. The present study showed that inflammatory responses were triggered in BV2 microglia cells activated by OGD/R, leading to the release of pro-inflammatory cytokines and apoptosis. DGMI suppressed the inflammatory response and apoptosis by regulating the TLR/MyD88/NF-κB signaling pathways and down-regulation of p-JNK1/2 and p-p38 MAPK activation.  
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    Nobiletin and tangeretin (citrus polymethoxyflavones): an overview on their chemistry, pharmacology and cytotoxic activities against breast cancer
    Eric Wei Chiang Chan, Oi Yoon Michelle Soo, Yong Hui Tan, Siu Kuin Wong, Hung Tuck Chan
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (7): 443-454.   DOI: 10.5246/jcps.2020.07.042
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    The fruit peel of Citrus species (Chenpi), particularly those of mandarin oranges, is a useful source of food and medicine in China. Flavonoids from the citrus fruit peel are mainly polymethoxyflavones (PMFs), of which nobiletin and tangeretin are the most abundant components. In the present review, we summarized the cytotoxic activities of these two PMFs to breast cancer cells. Studies have reported that these two compounds inhibit the growth of breast cancer cells by inducing apoptosis, cytostatic cell death and cell cycle arrest, or by inhibiting cell proliferation, metastasis and tumour angiogenesis, depending on the molecular subtypes of breast cancer. In vitro and in vivo cytotoxic activities involve different molecular targets and signalling pathways. Analyses on the structure-activity relationship (SAR) of nobiletin and tangeretin have shown that the presence of a methoxy group at C8 and a hydroxyl group at C3 or C5 are essential for anti-proliferative activity. Some future perspectives and research needs are suggested. Sources of information are from PubMed, PubMed Central, Science Direct, Google Scholar, J-Stage, PubChem and CNKI using keyword search.
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    Author Index of Volume 29
    Journal of Chinese Pharmaceutical Sciences
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 932-935.  
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    Comparative authentication of crude drugs from subgenus Asarum species in China based on microscopic features
    Yaoli Li, Zhong Liu, Jie Yu, Mingying Shang, Baibo Xie, Feng Xu, Shao-Qing Cai
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 855-867.   DOI: 10.5246/jcps.2020.12.076
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    According to the Chinese Pharmacopoeia, the Chinese medicinal material (Asari Radix et Rhizoma) Xixin is derived from the species Asarum heterotropoides var. mandshuricum, A. sieboldii, and A. sieboldii var. seoulense. However, many species of the genus Asarum are labeled as "Xixin", "Tu-Xixin" or "Nan-Xixin" for sale in producing areas. To distinguish Chinese Asarum species and to ensure their safety and efficacy, we examined the microscopic characteristics of subg. Asarum plants in China (including subg. Asarum plants in the classification system of Flora Reipublicae Popularis Sinicae and the later published Asarum yunnanense) using a hand microtome and a microscopic digital imaging system based on our previous research. Microscopic features of the blades, petioles, and roots of A. yunnanense were investigated for the first time. Moreover, we compared nine species and one variety of the subg. Asarum (i.e. , A. yunnanense, A. caudigerellum, A. himalaicum, A. pulchellum, A. debile, A. caulescens, A. renicordatum, A. caudigerum, A. caudigerum var. cardiophyllum and A. geophilum) in China based on microscopic characteristics of the blades. Several parameters, such as morphological characteristics and distribution of hairs on both upper and lower leaf epidermises; morphological characteristics of hair cells; anticlinal walls of lower epidermal cells; size relationship between outer periclinal walls and cavity outlines of oil cells on lower leaf epidermis; presence or absence of the irregular cells containing red to brown substances among mesophyll cells; and the diameter of the largest vessels in midribs of the blades, were used for discrimination among species. To the best of our knowledge, we provided the first identification key for the 10 taxa based on blade microscopic characteristics that could be used to distinguish these taxa. Based on the key, 23 commercial samples collected from seven provinces of China were authenticated as whole dried herbs of A. himalaicum, A. caudigerellum, A. caudigerum, A. pulchellum and A. caulescens. These findings indicated that microscopic approach and related techniques were convenient and feasible and could be used for the authentication of crude drugs from Chinese subg. Asarum species.

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    Significance of neutralizing antibodies in COVID-19 therapy: progress and prospect
    Zeqi Zhou, Xiangbin Wang, Xiqing Zhang, Yuan Zhang, Yankai Fu, Zhixian Wang, Yan Su, He Wang, Meng Xiao, Changxiao Liu
    Journal of Chinese Pharmaceutical Sciences    2021, 30 (2): 87-106.   DOI: 10.5246/jcps.2021.02.008
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    SARS-CoV-2 infection is a serious threat to human life and health all over the world, and COVID-19 is a global epidemic caused by SARS-CoV-2 infection. SARS-CoV-2 is highly infectious, strange and variable. Therefore, the treatment of COVID-19 must be urgent and targeted. However, vaccines and currently used drugs generally do not have the above-mentioned characteristics. Although convalescent plasma of COVID-19 has shown a clinical application value in the emergency treatment of critical patients, it shows great limitations. All human recombinant multivalent neutralizing nano-antibodies may meet the deficiency of COVID-19 therapy. Gene engineering technologies have been used to develop specific neutralizing antibody (nAB) drugs for the treatment of COVID-19 worldwide. Some of the candidate nAB drugs have been entered the clinical trials and can be used for the therapy of COVID-19 shortly. In the present review, we studied and analyzed nABs for the treatment of COVID-19 and the progress and prospect from the following five aspects: 1) The biological and clinical characteristics of SARS-CoV-2 infection; 2) The feasibility of plasma therapy for convalescents with COVID-19; 3) The technical routes of developing nAb drugs; 4) The current status of developing global COVID-19 antibodies; 5) The difficulties and clinical use.

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    Tao Liu research group of Peking University School of Pharmaceutical Sciences and Mo Li research group of Peking University Third Hospital joint developed CRISPR non-natural amino acid coupling technique to improve the efficiency of precise genome editing
    Shuxiang Song
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (5): 369-371.  
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    Tao Liu research group of Peking University School of Pharmaceutical Sciences and Mo Li research group of Peking University Third Hospital joint developed CRISPR non-natural amino acid coupling technique to improve the efficiency of precise genome editing.

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    Three new compounds from endophytic fungus Periconia sp. F-31
    Jimei Liu, Minghua Chen, Ridao Chen, Kebo Xie, Dawei Chen, Shuyi Si, Jungui Dai
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (4): 244-251.   DOI: 10.5246/jcps.2020.04.023
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    Three new compounds ( 1 3 ), including a chlorine-containing dihydroisocoumarin pericochlorosin A ( 1 ), a chlorinated phenol pericochlorosin B ( 2 ) and a decalin de rivative pericoannosin G ( 3 ), were isolated from endophytic fungus Periconia sp. F-31 of the medicinal plant Annona muricata . The structures and absolute configurations were elucidated by extensive spectroscopic methods and calculated electronic circular dichroism analysis. Compound 2 displayed potent anti-HIV activity with IC 50 value of 2.2 μM.
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    A biography of Academician Zhicen Lou
    Journal of Chinese Pharmaceutical Sciences
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 829-831.  
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    A biography of Academician Zhicen Lou.
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    Concomitant administration of gastric acid suppression might attenuates the clinical efficacy of gefitinib: a single cancer center retrospective study
    Zihan Guo, Qiong Du, Xuan Ye, Feifei Gao, Yufang You, Bo Yu, Qing Zhai
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (3): 192-198.   DOI: 10.5246/jcps.2020.03.016
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    R ecent pharmacokinetic studies have demonstrated that gastric acid suppression (AS) reduces exposure of gefitinib. Howeve r, the clinical significance of this drug-drug interaction (DDI) has not been determined. We, therefore, evaluated it in this real-world study. A total of 200 NSCLC patients who received gefitinib from 2016 to 2018 at Fudan University Shanghai Cancer Center (FUSCC) were randomly selected. The patients were divided into two groups according to whether AS was used. The clinical characteristics of the patients were collected, and the efficacy and safety of gefitinib were compared between the two groups. We showed that 188 patients were considered eligible for this retrospective analysis, 49 received AS (AS user group), while 139 patients did not (AS non-user group). Objective response rate (ORR) and disease control rate (DCR) in the AS user group versus A S non-user group were 69.4% versus 73.4% ( P = 0.591) and 89.8% versus 90.6% ( P = 0.486), respectively, while the progression-free survival (PFS) were 9.7 versus 12.2 months ( P = 0.0644). No significant difference in ORR, DCR or PFS was observed between the two groups. Further study showed that the PFS was related to the time of co-administration, and the patients receiving over 50% AS prescription overlap with gefitinib was significantly less compared with the other people (8.4 vs 12.6 months, P = 0.0004). The frequencies of rash (8.2% vs 15.1%, P = 0.281), diarrhea (4.1% vs 6.5%, P = 0.539) and elevated ALT or AST level (6.1% vs 10.1%, P = 0.407) were similar for both groups. Therefore, concomitant use of AS and gefitinib might affect the efficacy of gefitinib, which should be avoided if possible.  
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    The inhibitory activities of Urtica fissa to BPH
    Jin Huang, Lingxin Yang, Ning Yang, Bowen Yuan, Hao Zhang, Mengyue Wang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (4): 236-243.   DOI: 10.5246/jcps.2020.04.022
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    In the present study, the BPH inhibitory activities of Urtica fissa were systematically investigated. Firstly, inhibitory activities of 5 α reductase of the alcoholic extracts from different parts (root, stem, leaf, flower and fruit) were evaluated. The results indicated that the root possessed the most significant action. Subsequently, U. fissa root (UFR) was subjected to further pharmacological evaluation using the benign prostate hyperplasia (BPH) model rats induced by testosterone propionate. The results revealed that UFR could significantly decrease the prostate index, alter the hyperplasia tissue morphology, suppress the prostatic growth factors of VEGF, EGF , bFGF and KGF, decrease the inflammation factor levels of TNF- α , IL-1 β and IL-6, improve the activities of GSH-Px, CAT and SOD and decrease the MDA level in the prostate of the model rats. Moreover, UFR also significantly suppressed the hormone levels of testosterone and dihydrotestosterone. These results indicated that the possible BPH inhibitory mechanisms of UFR were growth factor suppression, hormone level modulation, anti-inflammation, and anti-oxidative stress.
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    Treatment analysis of acute fatty liver of pregnancy complicated with hematogenous infection of CRKP
    Lixia Li, Feng Chen, Xiaomei Xue, Fan Yang, Bin He
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (8): 591-595.   DOI: 10.5246/jcps.2020.08.055
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    Acute fatty liver of pregnancy (AFLP) is an idiopathic disease with acute and critical onset. Although the incidence of AFLP is relatively low, its mortality remains high. When AFLP is complicated with multi-organ dysfunction syndrome (MODS) and severe infection, especially hematogenous infection caused by multidrug-resistant pathogens, treatment becomes extremely difficult with an even lower survival rate. In the present work, we reported a 31-year-old primipara woman who developed AFLP complicated with MODS and severe infection at 37 +5 weeks of gestation and received treatment in our hospital. The result of fast blood culture was positive for carbapenem-resistant Klebsiella pneumonia (CRKP). Based on this finding in combination with the results of drug sensitivity test, the quadruple regime using scheme imipenem cilastatin sodium, tigecycline, polymyxin and fosfomycin was implemented, and the patient was successfully cured. Successful treatment of pregnant women with AFLP complicated with MODS and/or hematogenous infection of CRKP can be achieved by using individualized combined antibiotic therapies on the basis of fast blood culture and combined drug sensitivity test. In this case, the patient was in a critical situation. In the treatment process, clinicians, clinical pharmacists and microbiologists should cooperate with each other and discuss the treatment plan together according to the pathophysiological characteristics of the patient, which was one of the key factors for successful treatment of the patient.
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    Identification and discrimination of Ziziphi Spinosae Semen and Ziziphi Mauritianae Semen based on HPLC fingerprint analysis
    Hongshuai Yang, Qiuyi Liu, Weidong Yin, Manjiang Xie, Xiang Ji, Shuangyu Shao, Hong Liang, Pengfei Tu, Qingying Zhang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (4): 252-259.   DOI: 10.5246/jcps.2020.04.024
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    A simple, reproducible and reliable HPLC fingerprint analysis method of Ziziphi Spinosae Semen (ZSS) and Ziziphi M auritianae Semen (ZMS) was established, and then it was applied to analyze samples collected from different locations. A total of 12 common fingerprint peaks were designated in ZSS and ZMS, respectively, and five of which were definitely identified. Of these common peaks, 10 peaks were presented in both ZSS and ZMS, whereas two were characteristic for ZSS and ZMS, respectively. Furthermore, the two characteristic peaks of ZMS were definitely assigned as 6′′′-sinapoylspinosin and frangufoline by HPLC guided purification and structural elucidation . The HPLC fingerprint analysis was proved to be useful in identifying and discriminating ZSS and ZMS, which was beneficial for quality control of ZSS and its products.
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    Intestinal anti-inflammatory effects of main components of the fruits of Euodia rutaecarpa in a co-culture model of the human colorectal adenocarcinoma cells and RAW264.7 macrophages
    Shi Wang, Lu Liu, Youbo Zhang, Wei Xu, Xiuwei Yang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 868-879.   DOI: 10.5246/jcps.2020.12.077
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    Alkaloids are the main active constituents of the nearly ripe fruits of Euodia rutaecarpa (Juss.) Benth., which possesses antitumor, anti-inflammatory, analgesic and antimicrobial activities. In this study, we aimed to assess the potential anti-inflammatory effects of six main components, evodiamine, rutaecarpine, dehydroevodiamine, evocarpine, dihydroevocarpine and 1-methyl-2-undecyl-4-(1H)-quinolone, in the nearly ripe fruits of E. rutaecarpa. In the co-culture system consisting of the human colorectal adenocarcinoma (Caco-2) cells and RAW264.7 macrophages, inflammation was induced in RAW264.7 cells with 2 μg/mL lipopolysaccharide. The results indicated that evodiamine, rutaecarpine, and dehydroevodiamine significantly down-regulated the mRNA expressions of pro-inflammatory cytokines (Il-6, Il-1β and Tnf-α) and inflammatory mediators, such as cyclooxygenase-2 (Cox-2) and inducible nitric oxide synthase (iNos). Besides, the drug administration group showed a higher transepithelial electrical resistance and a lower tight junction permeability compared with the model group. Taken together, the tested compounds possessed anti-inflammatory effects. Our findings laid the foundation for further research on the anti-inflammatory mechanism of the nearly ripe fruits of E. rutaecarpa.

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    Protective effect of hydroxysafflor yellow A on Parkinson cell model and its network pharmacology analysis
    Shuang Li, Chunyan Guo, Shuangshuang Li, Sihan You, Xiujuan Yu
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 880-895.   DOI: 10.5246/jcps.2020.12.078
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    As an effective component of safflower, hydroxysafflor yellow A (HSYA) has the effect of promoting blood circulation, removing blood stasis, and relieving pain, and it has a certain effect on blood stasis and wind-induced Parkinson’s disease (PD). However, the current research mostly involves a single intervention mechanism, which is not conducive to the clinical transformation of this type of drugs. In the present study, rotenone was used to construct a PD cell model, and the protective effect of HSYA on the PD cell model was evaluated by cell viability and mitochondrial membrane potential. TTD database was used to query PD-related therapeutic targets, Swiss Target Prediction database to query HSYA-related targets, STRING database was used to search the gene interaction relationship of common targets, and ClueGO pathway was adopted to enrich and analyze common targets and their interaction targets, as well as to explore the comprehensive intervention mechanism. The results showed that rotenone could successfully establish the PD cell model, and HSYA had significant protective effect on PD cell model. Through the network pharmacological analysis, 36 PD-related therapeutic targets and 88 HSYA-related targets were queried. The common targets of PD and HSYA were FKBP1A, HTR1A, SLC6A4 and SLC6A3. To enrich four common targets and their interaction targets through REACTOME pathway, eight cell signal pathways were obtained, and six cell biological processes were obtained through biological process pathway enrichment.

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    A prospective cohort study on the relationship between vancomycin steady-state trough concentration and efficacy and safety in Chinese adults
    Chaohui Wu, Huifen Lin, Weiwei Lin, Yiwei Liu, Xiang You, Cuihong Lin, Rongfang Lin, Dayong Zeng, Pinfang Huang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (5): 341-354.   DOI: 10.5246/jcps.2020.05.032
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    Clinical guidelines recommend a steady-state vancomycin (VCM) trough concentration (SVTC) of 10–15 mg/L for regular infections and 15–20 mg/L for severe infections. However, clinical trials have shown that increasing SVTC is not beneficial for efficacy , and instead it leads to nephrotoxicity. To verify whether increasing the SVTC results in improved clinical outcomes with sustainable adverse effects, we prospectively determined its correlation with clinical efficacy and safety. The participants included patients hospitalized with Gram-positive bacterial infections from March 2017 through October 2018. The patients were classified into group I (SVTC<10 mg/L), II (10≤SVTC≤20 mg/L), or III (SVTC>20 mg/L). Clinical, microbiological, and laboratory data were collected. Clinical outcomes between group I and II were matched after propensity score matching (PSM). A total of 3 31 patients were included in this study. C linical failure occurred in 59 (29%) of 204 patients on day 14, with no significant difference between groups I and II ( P = 0. 535 ). Infection recurred at 28 d in 62 (30%) of 204 patients, and no significant difference in infection recurrence was observed between both the groups (log-rank, P = 0.674). Except for a significant increase in the incidence of acute kidney injury in group II, no significant difference was observed between two groups for any clinical result s . The incidence of adverse events in groups I and II was significantly lower than that in group III ( P <0.001). SVTC had an applicable cut-off point at 14.55 mg/L. SVTC was not correlated with VCM clinical efficacy, while it was a good indicator of nephrotoxicity .   
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    Pharmacological and clinical evaluation of a new anti-flu drug, baloxavir marboxil
    Jingjing Cao, Jun Sun, Shujuan Zhao, Peizhi Ma
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (10): 729-733.   DOI: 10.5246/jcps.2020.10.068
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    Baloxavir marboxil is a polymerase acidic (PA) endonuclease inhibitor, which is approved by the U.S Food and Drug Administration (FDA) on October 25, 2018, for the treatment of uncomplicated influenza patients aged 12 years and older. In the present work, we reviewed the pharmacodynamics, pharmacokinetics, drug interactions, clinical trials and adverse reactions of baloxavir marboxil .
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    Quality evaluation of Polyporus umbellatus based on HPLC specific chromatogram and fingerprint analysis
    Weijuan Jia, Shizhong Chen, Jianhua Sun, Zongli Bai, Yazhuo Huang, Yuankuan Zhang, Hong Wang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 896-907.   DOI: 10.5246/jcps.2020.12.079
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    To evaluate the quality of Polyporus umbellatus, we established a simple, repeatable and reliable method based on high performance liquid chromatography (HPLC) for specific chromatogram and fingerprint analysis, which was applied to analyze samples of medicinal materials and decoction pieces collected from different regions. Finally, ten characteristic peaks were designated in the specific chromatograms and applied to the authenticate identification of P. umbellatus samples. Nine common peaks were designated in the fingerprints, and then the similarities between 32 batches of samples were calculated. Among them, eight compounds were identified by HPLC-APCI-IT-TOF-MSn, four of which were identified in specific chromatograms and four in fingerprints. In the present study, we, for the first time, combined HPLC specific chromatograms and fingerprints for the species identification and quality evaluation of P. umbellatus. Collectively, our findings provided a new method for establishing a comprehensive quality standard of P. umbellatus.

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    Contents of Volume 29
    Journal of Chinese Pharmaceutical Sciences
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 915-927.  
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    Regulatory effects of traditional Chinese medicine on intestinal flora
    Sicong Tian, Jing Xue, Hui Song, Quan Du
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (3): 161-175.   DOI: 10.5246/jcps.2020.03.014
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    Two traditional Chinese medicines (TCMs), namely WangshiBaochiwan and Panax Notoginseng Saponins (notoginsenoside), were chosen to study their effects on gut microbiota. Both of them have a long history of application in China. During a test of 28 d, different doses of the medicines were administered to male Wistar rats daily. At the end of the administration, fresh fecal samples were collected and subjected to 16S rRNA sequencing to determine the profiles of gut microbiota. In relative to the controls, effects on gut microbiota were evaluated with medicine-treated rats. Consistent with its unique bidirectional effects on constipation and diarrhea, treatment of WangshiBaochiwan led to a balanced regulation of Lactobacillus and Bacteroides. The treatment also led to increased populations of Ruminiclostridium_9 and Eubacterium_ventriosum that are the major producer of short-chain fatty acid (SCFA), and decreased populations of genus Jeotgalicoccus and Bilophila that are associated with inflammatio n. These changes therefore resulted in a much healthier microbiota environment in WangshiBaochiwan-treated rates. For the treatment of notoginsenoside, effects were found with Enterobacteriaceae species that is associated with Parkinson's disease, Christensenellaceae family that is associated with aging, and hypertension-associated Rikenellaceae, Christensenellaceae, Lachnospiraceae and Bacteroidaceae species. In agreement with its major indications, the treatment further led to increased populations of SCFA-producing bacteria, such as Elusimicrobium, Anaerotruncus, Ruminococcaceae_NK4A214_group, and Intestinimonas. Taken together, treatment of the two TCMs led to active and distinguishable regulations of gut microbiota. Impressively, these changes were in agreement with their clinical efficacy, and suggested that they were involved in the treatment of these diseases.
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    Curcumin inhibits cervical cancer cell proliferation, migration and invasion by suppressing the PI3K/AKT signaling pathway via the miR-29b/KDM2A axis
    Xichun Guo, Jinghua Chen, Rujun Gao, Xiuyuan Han
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (6): 398-410.   DOI: 10.5246/jcps.2020.06.038
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    In this study, we aimed to examine whether curcumin exerted its anti-tumor effects by regulating miR-29b/KDM2A in cervical cancer cells. The cell viability, migration and invasion were estimated in HeLa cervical cancer cells treated with curcumin. The effects of microRNA-29b (miR-29b) on biological behaviors of HeLa SiHa cells were also assessed. Potential target genes of miR -29b were predicted and confirmed using a luciferase reporter assay, and the effects of curcumin and miR-29b on the PI3K/AKT signaling pathway were analyzed. Curcumin treatment inhibited cell proliferation, migration and invasion of HeLa cells ( P <0.05). The miR-29b expression was promoted by curcumin treatment in HeLa cells ( P <0.01), and miR-29b depletion could restore the effects of curcumin on cell proliferation, migration and invasion of HeLa cells ( P <0.05). KDM2A was proved as a direct target gene of miR-29b, and the activity of the PI3K/AKT signaling could be regulated by curcumin and miR-29b ( P <0.05). All the data revealed that curcumin played a protective role in cervical cancer. The proliferation, migration and invasion of cervical cancer cells were inhibited by curcumin through the miR-29b/KDM2A/PI3K/AKT pathway.
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    Antioxidantive activities and gastrodin determination of extracts of endophytic fungi isolated from Gastrodia elata Bl.
    Xiaoxia Liang, Cheng Xiong, Yingying Gao, Qiaojia Fan
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (3): 206-213.   DOI: 10.5246/jcps.2020.03.018
    Abstract38)   HTML0)    PDF (1366KB)(99)       Save
    Gastrodia elata Bl. is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness. However, its wild sources are now in short supply because of over-excavation. A total of 14 endophytic fungi were isolated from Gastrodia elata Bl collected from Yinjing, in which 10 strains were identified, including Neonectria , Ascomycota , Fusarium , Oidiodendron and Dothiorella . All crude extracts showed some degrees of antioxidant activities by T-AOC method and DPPH methods, especially those from Oidiodendron GeR6 and Ascomycota sp. GeR2 , which might be attributed due to their high phenolic contents. However, only Oidiodendron GeR6 contained gastrodin (39.2 μg/g) as the host plant in the crude extracts. In conclusion, the notable activities of GeR6 indicated its medical use as a natural antioxidant agent, as well as substitutable use for Gastrodia elata Bl. in the future.
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    Immobilization of β-glucuronidase: biocatalysis of glycyrrhizin to 18β-glycyrrhetinic acid and in-silico lead finding
    Makhmur Ahmad, Mohammad Rashid, Babar Ali, Shamshir Khan, Naseem Akhtar, Mohd Faiyaz Khan, Bibhu Prasad Panda
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (5): 333-340.   DOI: 10.5246/jcps.2020.05.031
    Abstract37)   HTML0)    PDF (3070KB)(95)       Save
    The non-covalent immobilization of β- glucuronidase enzyme obtained from Rhizopus oryzae was carried out by entrapment in natural fiber (papaya and coconut). The bioconversion capability of immobilized enzyme was analyzed based on conversion of glycyrrhizin to 18β-glycyrrhetinic acid under different conditions. The hydrolytic activity of the β-glucuronidase enzyme was highly depended on the microbial source and matrix, in which enzyme was immobilized. R. oryzae β-glucuronidase immobilized in papaya fibers produced the highest GA content (13.170 µg/mL) at 10 h of reaction. However R. oryzae β-glucuronidase immobilized in coconut fibers produced the highest GA content (21.425 µg/mL) at 15 h of reaction. Online Molinspiration software was used to predict drug like molecular properties of the 18β-glycyrrhetinic acid, and software suggested that the compounds had potential of becoming the orally active molecules. Therefore, in silico studies were conducted on proposed 18β-glycyrrhetinic acid to select the best possible drug candidates based on drug properties and bioactivity score of the compounds.
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    Urinary metabolomics analysis of the anti-depressive effects of Hemerocallis citrina extracts in a simulated microgravity-induced rat model of depression
    Teng Xu, Yan Wang, Cong Lu, Li Feng, Linxi Fan, Jing Sun, Bei Fan, Qiong Wang, Xinmin Liu, Fengzhong Wang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (3): 176-191.   DOI: 10.5246/jcps.2020.03.015
    Abstract36)   HTML0)    PDF (2083KB)(99)       Save
    We investigated the antidepressant-like activity of Hemerocallis citrine Baroni extract (HCE) in a simulated microgravity (SMG)-induced rat model of depression using a metabolomics method. A rat model, generated via 14 d of SMG induction, was validated from the reduced sucrose preference and the enhanced immobility time in the forced swimming test. HCE and paroxetine reversed certain metabolite profiles. Anti-depressant effects of HCE might involve the regulation of several metabolic pathways, such as phenylalanine, glutamic acid, and tryptophan metabolism and changes in energy metabolism. 5-Hydroxytryptophan, hippuric acid, phenylacetylglycine, citric acid, 3-hydroxykynurenine, cyclic AMP, and L-DOPA profiles were altered upon HCE and paroxetine administration. Furthermore, glutamic acid was only regulated in the HCE group, while xanthurenic acid and deoxyuridine were reversed in the positive group, suggesting differences in the mechanisms between the positive drugs and HCE in improving glutamic acid metabolism. This study provided a theoretical foundation for the application of HCE in depression therapy.
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    Integrating ancient and modern approaches to traditional Chinese medicinal authentication
    Zhongzhen Zhao, Hubiao Chen, Ping Guo, Zhitao Liang, Eric Brand, Lailai Wong, Jing Liu
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (12): 908-914.   DOI: 10.5246/jcps.2020.12.080
    Abstract36)   HTML16)    PDF (2085KB)(34)       Save

    Authentication is a key component of quality control, and the quality of Chinese medicinal materials directly impacts clinical efficacy. Chinese medicinal quality control is an important concern of the wider community, and it is intricately connected to the future and development of Chinese medicine. Macroscopic identification has been used for thousands of years in Chinese medicine, and it continues to be effective for resolving important issues related to quality control in the modern-day. However, new challenges have emerged for macroscopic identification. For example, new botanical varieties have emerged, with differences in macroscopic features related to cultivated vs. wild materials. Changes in processing methods and cultivation techniques also affect these features. Consequently, continual progress and innovation in Chinese medicinal authentication methods are needed. Macroscopic identification is based on organoleptic assessment, microscopy, and research of ancient texts, such as the Bencao. In addition to collecting information from traditional experience-based differentiation, innovation can take advantage of new technologies that can provide even more detailed, precise information about morphology. In the present review, we summarized inheritance and innovation in the scientific exposition of Chinese medicinal authentication, featuring a review of specialized publications, description of the establishment of a Chinese medicine specimen center and Chinese Medicine digital project, the expansion of authentication technologies, and the formation of a cultural project dedicated to the Compendium of Materia Medica.

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    Correlation between 5-hydroxymethylfurfural content and color of Rehmanniae Radix and Rehmanniae Radix Praeparata
    Cui Wu, Liang Xu, Bo Xu, Zhuojun Li, Zhimao Chao
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (5): 314-321.   DOI: 10.5246/jcps.2020.05.029
    Abstract35)   HTML0)    PDF (1869KB)(66)       Save
    In the present study, we explored the correlation between 5-hydroxymethylfurfural (5-HMF) and color of Rehmanniae Radix (RR) and Rehmanniae Radix Praeparata (RRP). The color was observed by human eyes under sunlight. The chromatic value and color difference were detected by a colorimeter. The water content was determined by an oven-drying method. The 5-HMF content was determined by an HPLC method. The correlation between the 5-HMF content and the color was analyzed by bivariate correlation analysis. The results showed that the color was greyish-brown and dark brown for RR, and it was black for RRP. The deepening color of RRP was reflected in the decrease of L * , a * and b * values. The water content was in accord with the requirement of Chinese Pharmacopoeia . The 5-HMF contents of RR and RRP were 0.9711–25.71 µg/g and 213.4–4010 µg/g, respectively. The average 5-HMF contents of RR and RRP were 8.059 µg/g and 1433 µg/g, respectively. The 5-HMF content in RRP was higher than that in RR. The correlations between 5-HMF content and L * , a * and b * values were significant. The color of RRP was deeper than that of RR. The 5-HMF content of RRP was higher than 0.02%, while it was less than 0.02% of RR, which could be used as a marker component for judging RR and RRP.
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    Preparative separation of high-purity troxerutin and related substances from mother liquor of troxerutin by silica gel column chromatography and semi-preparative liquid chromatography
    Shaojing Liu, Bei Qin, Hongfang Han, Li Li, Lili Yu, Xiaojing Xu
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (7): 487-493.   DOI: 10.5246/jcps.2020.07.046
    Abstract35)   HTML0)    PDF (5228KB)(21)       Save
    Troxerutin (TRO) is a mixture of semi-synthetic flavonoids prepared by hydroxyethylation of rutin, and it is commonly used for the treatment of cerebrovascular diseases. The main active ingredient is trishydroxyethyl rutin. The mother liquor of TRO contains a lot of TRO and other derivatives of hydroxyethylated rutin . In order to make full use of the mother liquor of TRO , an efficient method was developed for recovering high-purity TRO from mother liquor of TRO by combining silica gel column chromatography with semi-preparative liquid chromatography. In the silica gel column chromatographic separation, the ratio of silica gel to sample and eluent composition were investigated to obtain optimum separation effect. The results showed that when the ratio of silica gel to sample was 50, and acetone ethyl acetate water glacial acetic acid (10:10:3:1, v/v/v/v) was used as the eluent, the separation effect of TRO and adjacent impurities was good. Moreover, 150 g of TRO with a purity of 80% could be obtained from 1 kg of mother liquor of TRO by the silica gel column chromatographic separation, and the results were consistent with the quality standard of TRO raw material. Subsequently, the semi-preparative HPLC was performed, and 100 g TRO with a purity of up to 98% (w/w) was obtained. Meanwhile, tetrahydroxyethylrutin and tetrahydroxyethylquercetin with purity greater than 98% were obtained. This work proposed the separation and preparation of TRO with high-purity from the production waste of TRO for the first time, which had certain environmental benefits and economic benefits.  
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    Impact of the zero-markup drug policy on volume of medical service: based on 57 county hospitals of China
    Zhifeng Nie, Chunxia Man, Xin Yi, Yi Liu, Zhigang Guo, Xiaodong Guan, Luwen Shi
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (3): 220-226.   DOI: 10.5246/jcps.2020.03.020
    Abstract34)   HTML0)    PDF (1247KB)(76)       Save
    In the present study, we aimed to assess the long-term impact of zero-markup drug policy on volume of medical service in county hospitals of China. This study used 57 county hospitals’ records of medical service from 2011 to 2015, and a fixed effect model was applied to examine the impact of the zero-markup drug policy on volume of medical service. The outcome indicators included the number of patient visits, the number of prescriptions, the number of discharged patients, the length of stay, the number of patients who had surgeries and the number of patients who had CT or MRI, monthly. The number of hospitals which implemented the zero-markup drug policy was increased continuously. By the end of 2015, 41 hospitals (71.9%) implemented this policy. The panel regression showed that most indicators were insignificantly decreased, including the number of patient visits ( P <0.01), the length of stay ( P <0.05), the number of patients who had surgeries ( P <0.01) and the number of patients who had CT or MRI ( P <0.01). However, the number of prescriptions and the number of discharged patients were not significantly changed. The implementation of the zero-markup drug policy might affect the reduction of the volume of medical service in county-level hospitals and the reason needs to be clarified in future studies.  
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    Determination of β-amyloid peptides in vitro aggregation process by electrochemistry
    Sufang Zhang, Cong Li, Ying Zhang, Juncheng Zou, Yinzhu Cui, Xiaomei Ling
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (3): 199-205.   DOI: 10.5246/jcps.2020.03.017
    Abstract33)   HTML0)    PDF (1704KB)(87)       Save
    An impedimetric sensor (EA/mAb/CeZnO/GCE) for detecting β-amyloid peptides (Aβ) was successfully constructed by synthesizing ceria doped zinc oxide (CeZnO) nanoparticles to modify the glassy carbon electrode (GCE) for immobilizing the antibody mAb, and blocking the unbound carboxyl sites with ethanolamine (EA). The sensor exhibited a linear detection range of 10–100 pM and a detection limit of 1.7 pM (S/N = 3), and the relative standard deviations (RSDs) of the inter-electrode and intra-electrodes were less than 3.0%. After it was stored at 4 °C for 2 d, its impedance value was 101.6% of original impedance response. The sensor was used to measure the remaining content of Aβ incubated in vitro . The results showed that after incubation for 48 h and 60 h, the content of aggregated Aβ (oligomers and fibers) almost unchanged, reaching about 90%. This method had the advantages of time-saving, simple procedure, economical, excellent conductivity, good reproducibility and stability.   
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    Phytochemistry, biological properties and quality control of Chuanxiong Rhizoma: a review
    Jinhui Shi, Ruiyue Li, Siyu Yang, Hongmei Zhang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (11): 755-779.   DOI: 10.5246/jcps.2020.11.070
    Abstract33)   HTML14)    PDF (1995KB)(44)       Save
    Chuanxiong Rhizoma is a widely used Chinese herbal medicine in the past 2000 years. Chuanxiong Rhizoma is composed of volatile oils, phthalide lactones, phenolic acids, polysaccharides and other compounds. To date, more than 149 compounds in Chuanxiong Rhizoma have been isolated and identified, and some of them have been reported to possess promising biological properties on cardiovascular and central nervous system disorders besides their anti-cancer and antioxidant effects. Modulation of inflammatory mediators and apoptotic factors are believed to contribute to its bioactivities. Analytical methods, such as HPLC, GC and UPLC, are employed for qualitative evaluation of Chuanxiong Rhizoma. In this work, harvest period, growing habitat, processing method and storage, which can affect the quality of Chuanxiong Rhizoma, were also discussed. Comprehensive quality control methods should be developed to ensure the safety, quality and efficacy use of Chuanxiong Rhizoma. Herein, we collected and analyzed the literature of Chuanxiong Rhizoma published on CNKI, ScienceDirect, Springer link, Wiley and PubMed in past two decades, and up-t o-date information of Chuanxiong Rhizoma was provided in this paper. We suggested ligustilide, bu tylidenephthalide and total senkyunolides as the chemical markers to evaluate the quality of Chuanxiong Rhizoma. Additionally, the influences of soil conditions and processing methods on Chuanxiong Rhizoma as future research perspectives should also be further assessed.
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    Detection of minor constituents in TongMai Granules using NP×RP off-line two-dimensional liquid chromatography coupled with orbitrap mass spectrometry
    Lulu Xu, Zhanpeng Shang, Yue Chai, Yang Zhang, Rong Yu, Min Ye, Xue Qiao
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (11): 793-803.   DOI: 10.5246/jcps.2020.11.072
    Abstract32)   HTML12)    PDF (2682KB)(39)       Save
    TongMai Granules is a Chinese medicine patent drug to treat cardiovascular diseases. It is prepared from the water extract of Dan-shen, Chuan-xiong and Ge-gen. The main compounds of TongMai Granules are isoflavone glycosides, isoflavones, and phenolic acids. However, it is difficult to detect low-polar compounds such as tanshinones and phthalides though they may also contribute to the therapeutic effect. In this work, an off-line two-dimensional chromatography method was developed to enrich the minor constituents in TongMai Granules. The extract was subjected to an SEP-PAK silica SPE column and eluted with petroleum ether, ethyl acetate–methanol ( 6:1 , v/v), and methanol, successively. The three fractions were then analyzed using ultra-high performance liquid chromatography coupled with orbitrap mass spectrometry (UHPLC/orbitrap-MS). The isoflavones from Ge-gen, phenolic acids from Dan-shen and phthalides from Chuan-xiong were distributed in different fractions. A total of 131 compounds, including 59 from Ge-gen, 26 from Dan-shen, 43 from Chuan-xiong, and 3 common ones from Dan-shen and Chuan-xiong were tentatively identified. Among them, 26 compounds were identified by comparing with reference standards. This study set a good example for comprehensive analysis of the constituents in traditional Chinese medicine formula.
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    Microwave-assisted preparation and in vitro characterizations of doped superparamagnetic ferrite nanoclusters
    Xiaoxin Liu, Xiaoya Qin, Ziyuan Li, Tianyuan Fan
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (3): 153-160.   DOI: 10.5246/jcps.2020.03.013
    Abstract31)   HTML0)    PDF (1911KB)(102)       Save
    In order to obtain nanomaterials with superparamagnetism and high saturation magnetization, Mn-doped or Zn-doped superparamagnetic ferrite nanoclusters (Mn-FNs or Zn-FNs) were prepared by microwave-assisted solvothermal method in this study. Preliminary investigations were performed by transmission electron microscopy (TEM) and dynamic light scattering (DLS) instrument to observe the morphology and measure the particle size, respectively. Afterwards, Zn-FNs were chosen to be further characterized in vitro due to their better morphology and dispersity than Mn-FNs. The subsequent characterizations included crystalline phase, metal content and magnetic properties by X-ray diffractometer (XRD), inductively coupled plasma-mass spectrometry (ICP-MS) a nd vibrating sample magnetometer (VSM), respectively. The results showed that Zn-FNs had a cluster-like structure assembled by multiple nanoparticles. Zn-FNs were spherical in shape with good dispersity and relatively uniform particle size. Zn was successfully doped in Zn-FNs which demonstrated spinel structure and excellent magnetic properties. Therefore, Zn-FNs had a favorable application prospect as a new type of magnetic nanomaterial.  
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    Chemical constituents from Trichosanthes cucumeroides
    Jing Chen, Li Cheng, Fuqian Wang, Xuanbin Wang, Yonghui Zhang, Xincai Hao
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (6): 431-438.   DOI: 10.5246/jcps.2020.06.041
    Abstract31)   HTML0)    PDF (1425KB)(44)       Save
    One new triterpenoid, 3 β -[(E)-caffeoyloxy]-D:C-friedooleana-7,9(11)-dien-29-oic acid ( 1 ) and nine known triterpenoids identified as cucurbitacin B ( 2 ), 23,24-dihydrocucurbitacin B ( 3 ), 23,24-dihydrocu-curbitacin D ( 4 ), cucurbitacin D ( 5 ), cucurbalsaminol A ( 6 ), 3-epi-isocucurbitacin D ( 7 ), curbitacin G ( 8 ), cucurbitacin J ( 9 ) and cucurbitacin I ( 10 ) were purified from Trichosanthes cucumeroides . Their structures were confirmed by spectroscopic data analysis and comparison with previous literature. All compounds were obtained from T. cucumeroides for the first time.   
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    Virtual screening for triple-negative breast cancer cell inhibitors based on telomere G-quadruplex structure
    Hua Deng, Chao Gao, Dengguo Wei, Sisi Liu
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (6): 383-389.   DOI: 10.5246/jcps.2020.06.036
    Abstract30)   HTML0)    PDF (1326KB)(81)       Save
    Triple-negative breast cancer is an aggressive subtype that frequently develops resistance to chemotherapy. It is expected to develop new anti-tumor drugs through targeting the structure of G-quadruplexes of the genes associated with this tumo r. In this work, by targeting the 21-mer telomere G-quadruplex structure, compounds VB07 and VC02 were identified to stabilize the telomere G-quadruplex through structure-based high-throughput virtual screening . Cell cytotoxicity assay showed that VB07 and VC02 exhibited inhibitory effect on triple-negative breast cancer cells at the concentration of 5 μM. This study showed that structure-based high-throughput virtual screening was able to successfully identify the proper compounds targeting the telomere G-quadruplex, which exhibited inhibitory effects against the t riple-negative breast cancer cells.  
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    Discussion of the process control of large-scale instruments and equipment procurement in State Key Laboratory
    Shuxiang Song
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (3): 214-219.   DOI: 10.5246/jcps.2020.03.019
    Abstract30)   HTML0)    PDF (1117KB)(89)       Save
    In recent years, the usage, management and benefit of large-scale scientific research instruments and equipment in scientific research institutes have been a leading issue in the management of scientific research institutes . Within the scope of equipment budget, it is necessary for each equipment acquisition team to conduct a round of communication, coordination and negotiation with suppliers in order to improve the cost performance of equipment procurement and maximize the performance index to meet the needs of scientific research. By introducing the practical experience of the State Key Laboratory in purchasing imported equipment and managing large-scale instruments, this paper probes into the management process of the imported large-scale scientific research tax-free equipment of scientific research institutes, and explores the system and methods to guarantee and improve the efficiency of large-scale instruments in scientific research institutes from the aspects of policy, funds and technology .
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    Chemical comparison of Semen Euphorbiae and Semen Euphorbiae Pulveratum by UPLC-Q-TOF/MS coupled with multivariate statistical techniques
    Huinan Wang, Jingzhen Zhang, Yuexin Cui, Siyu Wang, Hui Gao, Yao Zhang, Xinjie Wang, Ziye Yang, Mengyu Chen, Peihua Wang, Guimei Zhang, Yingzi Wang, Chao Zhang
    Journal of Chinese Pharmaceutical Sciences    2020, 29 (7): 470-479.   DOI: 10.5246/jcps.2020.07.044
    Abstract30)   HTML0)    PDF (5459KB)(101)       Save
    In the present study, we aimed to assess the chemical composition changes of Semen Euphorbiae (SE) and Semen Euphorbiae Pulveratum (SEP) by UPLC-Q-TOF/MS and multivariate statistical methods. The UPLC-Q-TOF/MS method and SIMCA-P software were used to analyze the changes of chemical components of SE and SEP based on PCA and PLS-DA multivariate statistical methods. A “component-target-disease” network model was constructed by Intelligent Platform for Life Sciences of traditional Chinese medicine (TCM) to predict potential related diseases. The differences of chemical composition were significant between SE and SEP. Under positive ion mode, the amounts of Euphorbia factor L 2 , L 3 , L 7a , L 8 , L 9 and lathyrol were obviously decreased after processing. Under negative ion mode, the amounts of aesculetin, bisaesculetin, ingenol and cetylic acid were increased obviously, while Euphorbia factor L 1 , L 4 and L 5 were decreased obviously after processing, and the components of euphobiasteroi d, aesculetin, lathyrol and linoleic acid among the 14 differentiated compounds were closely related to the SE-related diseases through the “component-target-disease” network model. UPLC-Q-TOF/MS technology in combination with multivariate statistical methods had certain advantages in studying the complex changes of chemical composition before and after manufacturing pulveratum of SE. It provided a basis for clarifying the toxicity-attenuated mechanisms of SE manufacturing pulveratum, and laid the foundation for its further development and utilization.  
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