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Journal of Chinese Pharmaceutical Sciences ›› 2018, Vol. 27 ›› Issue (12): 840-846.DOI: 10.5246/jcps.2018.12.085

• Original articles • Previous Articles     Next Articles

Anti-hyperlipidaemic assay and LC-MS/MS determination of piperinic ester in rat

Bo Surina1, Dan Mu1, Ochir Sarangua2, Chunjie Ma3*   

  1. 1. College of Pharmacy, Inner Mongolia Medical University, Inner Mongolia, Jinshan Development Zone, Hohhot 010110, China
    2. Academy of Mongolian Medicine, Inner Mongolia Medical University, Inner Mongolia, Jinshan Development Zone, Hohhot 010110, China
    3. College of traditional Chinese medicine, Inner Mongolia medical University, Inner Mongolia, Jinshan Development Zone, Hohhot 010110, China
  • Received:2018-07-11 Revised:2018-08-02 Online:2018-12-30 Published:2018-11-05
  • Contact: Tel.: +86-13514819729, E-mail: 13514819729@163.com
  • Supported by:

    Clinical Innovative Team of Chinese and Mongolian Medicine (Grant No. YKD2007KJCXTD008).

Abstract:

In the present study, we aimed to establish high-fat diet model for evaluating anti-hyperlipidaemic activity of GBA and to establish the HPLC-MS method for measuring the pharmacokinetics of GBA in rat blood plasma and liver after oral administration. Rats were orally administrated with GBA at the dose of 50mg/kg body weight. GBA concentrations in blood plasma and liver were detected by HPLC from 0.510 h post administrations. The pharmacokinetic parameters were analyzed by the DAS1.0Software. The concentration-time curves of the plasma GBA and liver GBA wereconformed to two-compartment model. GBA was rapidly absorbed in the gastrointestinal tract and could be detected in plasma and liver 0.5 h after oral administration.

Key words: Long pepper, Piperinic ester GBA, Antihyperlipidaemic activity, LC-MS/MS analysis

CLC Number: 

Supporting: