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Journal of Chinese Pharmaceutical Sciences ›› 2016, Vol. 25 ›› Issue (12): 898-905.DOI: 10.5246/jcps.2016.12.101

• Original articles • Previous Articles     Next Articles

Chemical constituents from Portulaca oleracea and their bioactivities

Tianyun Jin, Tao Shen, Mingxing Zhou, Ailing Li, Da Feng, Bolun Zheng, Jie Gong, Jiawei Sun, Lingyu Li, Lan Xiang*   

  1. Department of Pharmacognosy, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
  • Received:2016-06-22 Revised:2016-09-19 Online:2016-12-21 Published:2016-10-18
  • Contact: Tel.: +86-0531-88382028, E-mail: xianglan02@sdu.edu.cn
  • Supported by:

    National Natural Science Foundation of China (Grant No. 81073005), Science and Technology Development Program of Shandong Province (Grant No. 2014GSF119007), Major Project of Science and Technology of Shandong Province (Grant No. 2015ZDJS04001), Young Scholars Program of Shandong University (Grant No. YSPSDU2015WLJH50), and China-Australia Centre for Health Sciences Research (2015).

Abstract:

In the present study, we aimed to intensively study the chemical constituents, especially organic acids from a medicinal plant Portulaca oleracea L., and screen their anti-inflammatory and quinone reductase (QR, a phase II detoxyfication enzyme) inductive activity. A total of 20 compounds were isolated and identified based on spectroscopic methods, as succinic acid (1), mono-methyl succinate (2), L-malic acid (3), L-1-methyl malate (4), L-4-methyl malate (5), L-dimethyl malate (6), L-6-ethyl citrate (7), L-1-methyl citrate (8), L-1,5-dimethyl citrate (9), 4-hydroxy-5-methylfuran-3-carboxylic acid (10), 5-hydroxymethyl-furoic acid (11), stearic acid (12), L-pyroglutamic acid (13), cyclo-(tyrosine-leucine) (14), L-isoleucine (15), (–)-dehydrovomifoliol (16), ()-epiloliolide (17), 3,4-dihydroxyphenylethanol (18), succinimide (19), and uracil (20). Among them, 14 compounds (2, 48, 10, 11, 1318) were isolated from P. oleracea for the first time. Compound 18 (12.5 μM) exhibited potent anti-inflammatory effect in lipopolysaccharide (LPS)-induced macrophage cells (RAW264.7) by reducing NO production, and it also increased QR activity in Hepa lclc7 cells. Compound 16 (50 μM) showed weak QR inductive activity. None of other compounds showed anti-inflammatory or QR inductive activities.

Key words: Portulaca oleracea, Chemical constituents, Anti-inflammatory, QR inductive activity

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