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Journal of Chinese Pharmaceutical Sciences ›› 2016, Vol. 25 ›› Issue (9): 651-659.DOI: 10.5246/jcps.2016.09.072

• Original articles • Previous Articles     Next Articles

The adsorption of cellular proteins affects the uptake and cellular distribution of gold nanoparticles

Mengmeng Qin, Yifan Li, Bing He, Bei Wei, Wenbing Dai, Hua Zhang, Xueqing Wang, Qiang Zhang*   

  1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2016-04-07 Revised:2016-05-03 Online:2016-09-28 Published:2016-05-15
  • Contact: Tel.: +86-010-82802791, E-mail: zqdodo@bjmu.edu.cn
  • Supported by:

    The National Basic Research Program of China (973 program, Grant No. 2015CB932100) & National Natural Science Foundation of China (Grant No. 81130059).

Abstract:

Nanotechnology has been widely used in the field of medicine, and it can significantly improve the bioavailability and the target efficiency of medicines. However, after administration, nanomedicines can adsorb biomolecules that can influence their effects. It was reported that the adsorption of plasma proteins can change the surface properties of nanoparticles. When nanoparticles pass through cells, they may carry some cellular proteins out of cells. Currently, it is unclear whether the adsorbed proteins affect the uptake of nanoparticles in the next cell layer. To simplify this complex biological process, BSA-capped gold nanoparticles were prepared and incubated with Caco-2 cell lysate to simulate conditions of transcytosis through epithelial cells. The surface morphology of nanoparticles was examined by TEM. SRB was used to evaluate the cytotoxicity of the nanoparticles. The uptake and cellular distribution of the nanoparticles were detected by ICP-MS and CLSM. The results suggested that the adsorption of cell proteins could enhance the adhesion and uptake of gold nanoparticles. The gold nanoparticles were mainly located in lysosomes, and there were some Lysate-capped AuNPs in the mitochondria whereas no BSA-capped AuNPs appeared there.

Key words: Gold nanoparticles, Proteins, Cell lysate, Adsorption, Enhance, Cellular uptake

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