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Journal of Chinese Pharmaceutical Sciences ›› 2016, Vol. 25 ›› Issue (8): 590-597.DOI: 10.5246/jcps.2016.08.066

• Original articles • Previous Articles     Next Articles

TRHH-herb pair prevents IL-1β-induced degeneration of endplate chondrocytes in vitro

Kai Niu1,2,4, Chenguang Li2,3, Song Yuan1, Lei Zhang2, Qi Shi3,4, Yongjun Wang3,4, Weichao Zheng2*   

  1. 1. Second People’s Hospital of Zhoushan, Zhoushan 316000, China
    2. Medical College, Anhui University of Science and Technology, Huainan 232001, China
    3. Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
    4. Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China
  • Received:2015-09-21 Revised:2015-10-13 Online:2016-09-01 Published:2015-10-27
  • Contact: Tel./Fax: +86-0554-6668808, +86-0580-2363011, E-mail: nk342896716@126.com, wzheng@aust.edu.cn
  • Supported by:

    National Natural Science Foundation of China (Grant No. 81273777, 81102606, 81072831), the National Basic Research Program of China (Grant No. 2010CB530400), the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT, IRT1270).

Abstract:

The inflammatory cytokine interleukin-1 beta (IL-1β) plays a key role in the process of intervertebral disc degeneration (IVDD). In the present study, we aimed to evaluate the effect of pharmaco-serum of "Taoren-Honghua-herb pair" on IL-1β-induced chondrocyte degeneration in vitro. Taoren (Semen persicae) and Honghua (Safflower carthamus) were administered to the rats, and the pharmaco-serum was collected and prepared. Chondrocytes of the third passage, isolated from the rat’s vertebral endplates, were treated by standard medium only (Group NC), IL-1β (Group IL) or combination of IL-1β and pharmaco-serum (Group TRHH). Cell proliferation and apoptosis were determined, and the expression of aggrecan, Col2α1, Col10α1, IL-6 and SOX9 at the mRNA level in chondrocytes was quantified by real-time PCR. Immunohistochemistry staining of type II and X collagen and Safranine O staining were also used to evaluate the chondrocytes. Compared with the Group NC, IL-1β treatment inhibited the cell proliferation and induced the cell apoptosis (P<0.05), and the expression of aggrecan, Col2α1 and SOX9 at the mRNA level was down-regulated. In contrast, the expression of Col10α1 and IL-6 was up-regulated after IL-1β treatment (P<0.05). Meanwhile, the immune-staining of type II collagen and Safranine O staining were decreased, while the staining of type X collagen was increased. Compared with the Group IL, cell proliferation was increased, and apoptosis of chondrocytes was decreased when cells were treated with the pharmaco-serum of TRHH-herb pair (P<0.05). The expression of aggrecan, Col2α1 and SOX9 at the mRNA level was up-regulated, while that of Col10α1 and IL-6 was down-regulated (P<0.05). Safranine O staining also showed increased positive staining (P<0.05). Taken together, the treatment of pharmaco-serum of TRHH-herb pair could prevent endplate chondrocyte degeneration induced by IL-1β.

Key words: Pharmaco-serum, TRHH-herb pair, Degeneration, Chondrocyte, Intervertebral discs

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