Journal of Chinese Pharmaceutical Sciences ›› 2016, Vol. 25 ›› Issue (5): 380-386.DOI: 10.5246/jcps.2016.05.043

• Original articles • Previous Articles     Next Articles

An UPLC-MS/MS application to investigate the chemical composition of the ethanol extract from Anoectochilus chapaensis and its hypoglycemic activity in insulin-resistant HepG2 cells

Cai Jinyan1*, Ni Jun1, Zhao Lin2   

  1. 1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China;
    2. School of Life Science and Bio-pharmaceutical, Guangdong Pharmaceutical University, Guangzhou 510006, China
  • Received:2016-02-23 Revised:2016-03-25 Online:2016-05-30 Published:2016-04-12
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  • Supported by:

    Pearl River Nova Program of Guangzhou 2015 (Grant No. 201506010061), Foundation for Distinguished Young Teachers in Higher Education of Guangdong (Grant No. YQ2015097), and National Natural Science Foundation of China (Grant No. 81001628).


Anoectochilus chapaensis Gagnep. (Orchidaceae) was named as the “king of medicine” because of its excellent efficacy for the treatment of diabetes. However, the bioactive constituents are unknown. An ethanol extract from A. chapaensis showed significant stimulating effect on glucose consumption in HepG2 cells. The chemical composition was investigated by UPLC-MS/MS in negative electrospray ionization (ESI) mode, and 63 compounds including flavonoids, triterpenoids, and aliphatic acids were tentatively identified by accurate mass and characteristic fragments. Moreover, the method of hypoglycemic screening with insulinresistant HepG2 cells and UPLC-MS/MS might be potentially useful in rapid and efficient characterization and primary prediction of natural products prior to traditional isolation.

Key words: UPLC-MS/MS, Hypoglycemic, HepG2 cells, Anoectochilus chapaensis

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