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Journal of Chinese Pharmaceutical Sciences ›› 2016, Vol. 25 ›› Issue (5): 366-372.DOI: 10.5246/jcps.2016.05.041

• Original articles • Previous Articles     Next Articles

Albiflorin attenuates inflammatory injury by regulating the TLR4 signaling pathway and its negative regulating factor Tollip in experimental models of ulcerative colitis

Xinhua Fang1, Xin Wu2, Xuemei Zhu3, Hao Cai4, Gang Cao2*   

  1. 1. TCM Dispensary, Traditional Chinese Medical Hospital of Hangzhou, Hangzhou 310007, China
    2. Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China
    3. Department of Pharmacy, Wenzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, Wenzhou, 325000 China
    4. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
  • Received:2016-01-24 Revised:2016-02-15 Online:2016-05-30 Published:2016-03-12
  • Contact: Tel.: +86-571-87195895, E-mail: caogang33@163.com
  • Supported by:

    National Natural Science Foundation of China (Grant No. 81573602), the Project of Science and Technology for Chinese Medicine of Zhejiang Province, China (Grant No. 2013KYB183), the Chinese Medicine Research Program of Zhejiang Province, China (Grant No. 2014ZQ008, 2016ZA183, 2015ZQ011), and Zhejiang Province Association of Traditional Chinese Medcine (Grant No. 2013103).

Abstract:

Albiflorin (AF) is the main active component extracted from Paeoniae Radix Alba. This study investigated the efficacyof AF in attenuating inflammatory injury by regulating the TLR4 signaling pathway and its negative regulating factor Tollip in an experimental ulcerative colitis (UC) model. We administrated trinitrobenzene sulfonic acid for 21 d to induce UC in rats. The efficacy of AF in attenuating UC was assessed using various biochemical markers, such as tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-10 (IL-10), 5-hydroxytryptamine (5-HT), and tissue myeloperoxidase (MPO), along with histopathological studies on toll-like receptor-4 (TLR-4) signaling pathway and its negative regulating factor Tollip. The results showed that AF can significantly downregulate the levels of TNF-α, IL-1, IL-10, and 5-HT. AF decreased the activation of TLR4, MyD88, and NF-κB p65 protein expression by increasing Tollip expression. AF can relieve symptoms of UC by suppressing the activation of the TLR4 signaling pathway and upregulating its negative regulating factor Tollip. Therefore, AF may be a potential natural product for treating UC.

Key words: Albiflorin, Ulcerative colitis, TLR4 signaling pathway, Tollip

CLC Number: 

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