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Journal of Chinese Pharmaceutical Sciences ›› 2016, Vol. 25 ›› Issue (4): 275-283.DOI: 10.5246/jcps.2016.04.031

• Original articles • Previous Articles     Next Articles

HPLC determination of Nimodipine in plasma with an improved sample refining method and its application in pharmacokinetic studies

Xiaona Wang, Ruilian Chen, Wenli Liu, Junbo Gong, Yongli Wang, Zhenping Wei*   

  1. Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • Received:2015-12-28 Revised:2016-01-25 Online:2016-04-21 Published:2016-01-29
  • Contact: Tel.: +0086-22-27890336, E-mail: zpwei2000@sina.com
  • Supported by:

    National Natural Science Foundation of China (Grant No. 21176173) and Tianjin Natural Science Foundation (Grant No.14JCYBJC29100).

Abstract:

In order to prepare samples for HPLC analysis with maximum drug recovery and impurity elimination, a revised method for the extraction and purification of a target substance from plasma was developed and applied in a pharmacokinetic study with Nimodipine as a model drug. After protein precipitation of a plasma sample using pure methanol and evaporation of the supernatant to dryness, methanol of various concentrations from 10% to 100% were used to dissolve the remaining residues with the goal of maximizing drug recovery and impurity elimination. Through rigorous screening with HPLC peaks from residual impurity and recovered drug as the criteria, a methanol concentration of 30% was chosen. The standard curve was linear (r2≥ 0.999) over the range of 2–160 ng/mL with a limit of quantification (LOQ) of 2 ng/mL. Intra- and inter-day precision values were below 15%, and the accuracy ranged from –1.70% to 5.88% at all three quality control (QC) levels. The wavelength of maximum absorption was 238 nm, and a smaller LOQ value of 2 ng/mL was achieved compared with the reported method. The revised method was successfully applied in a pharmacokinetic study of Nimodipine in rats and sample preparations of lidocaine hydrochloride.        

Key words: Sample purification, Nimodipine, HPLC

CLC Number: 

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