Journal of Chinese Pharmaceutical Sciences ›› 2016, Vol. 25 ›› Issue (2): 116-121.DOI: 10.5246/jcps.2016.02.012

• 【Original articles】 • Previous Articles     Next Articles

An UPLC-MS/MS application to investigate chemical compositions in the ethanol extract with hypoglycemic activity from Zingiber striolatum Diels

Tianhong Chen, Jinyan Cai*, Jun Ni, Fan Yang*   

  1. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China
  • Received:2015-09-17 Revised:2015-11-11 Online:2016-02-29 Published:2015-11-18
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  • Supported by:

    Pearl River S&T Nova Program of Guangzhou (Grant No. 201506010061), Foundation for Distinguished Young Teachers in Higher Education of Guangdong, and special funds for cultivating Guangdong college students’ scientific and technological innovation (“Climbing plan”, Grant No. pdjh2015b0277).


Zingiber striolatum Diels (Zingiberaceae) is an edible plant resource in the Chinese folk with special efficacy in relieving diabetes and constipation, whichhas been documented in the Compendium of Materia Medica. However, its hypoglycemic activity and constituents have not been reported yet. In the present study, we evaluated the hypoglycemic activity of Z. striolatum in insulin-resistant HepG2 cells, and we developed ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) based chemical profiling method for rapid analysis of Z. striolatum. As a result, the ethanol extract from Z. striolatum showed significant hypoglycemic activity in HepG2 cells, and 22 flavonoids compounds were tentatively characterizedby comparing the retention time and mass spectrometry data. In conclusion, the method of hypoglycemic screening in insulin-resistantHepG2 cells coupled with UPLC-MS/MS is a feasible and credible technique to separate and identify the active constituents in complex matrices of traditional Chinese medicine.

Key words: Hypoglycemic activity, HepG2 cells, UPLC-MS/MS, Zingiber striolatum Diels

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