Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (9): 642-648.DOI: 10.5246/jcps.2014.09.082

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Simultaneous determination of seven caffeoylquinic acids and three flavonoids in Pyrrosia petiolosa (Christ) Ching by RP-UFLC-DAD

Biao Ren, Hongyang Sun, Hong Wang*   

  1. School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2014-05-23 Revised:2014-05-27 Online:2014-09-23 Published:2014-06-10
  • Contact: Tel.: 86-10-82801559, 86-10-82802723
  • Supported by:
    Study of Safety Testing Techniques and Standards on New Traditional Chinese Drug (National Key Science and Technology Special Projects, Grant No. 2014ZX09304307-001-001).


An efficient, sensitive, accurate and rapid analytical ultra-fast liquid chromatography (UFLC) method for quality evaluations of Pyrrosia petiolosa (Christ) Ching from 20 regions of China was developed in this study. Ten marker compounds were simultaneously quantified, including 5-caffeoylquinic acid (5-CQA), 3-caffeoylquinic acid (3-CQA), 4-caffeoylquinic acid (4-CQA), 1-caffeoylquinic acid (1-CQA), 3,5-dicaffeoylquinic acid (3,5-diCQA), 4,5-dicaffeoylquinic acid (4,5-diCQA), 3,4-dicaffeoylquinic acid (3,4-diCQA), astragalin, kaempferol-3,7-di-O-glucoside and (±)eriodictyol-7-O-β-D-glucuronide. Chromatographywas performed on a Kromasil 100-2.5C18 (100 mm×2.1 mm, 2.5 μm) C18 column with gradient elution. The mobile phases consisted of 0.1% formic acid/water (A) and 0.1% formic acid/methanol (B). The detection wavelength was set at 326 nm and the flow rate was 0.4 mL/min. Ten components were separated well with good linearity (r2>0.9998), precision, repeatability, stability. The recovery was in the range of 99.08%-102.77%. The results showed that the content determination using RP-UFLC-DAD fingerprint technique provides an efficient, sensitive, accurate and rapid analytical method for quality assessment of P. petiolosa (Christ)Ching. Cluster analysis and principal components analysis were successfully applied to analyze 20 samples, the results revealed that the method was efficient and authentic to distinguish producing areas and the source of P. petiolosa (Christ) Ching.

Key words: Pyrrosia petiolosa (Christ) Ching, Caffeoylquinic acids, Flavonoids, Multicomponent determination, UFLC

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