Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (8): 525-532.DOI: 10.5246/jcps.2014.08.068

• Original articles • Previous Articles     Next Articles

Isolation and characterization of cyclic lipopeptides from the marine-derived Bacillus licheniformis

Du Gao1, Li Tian2,3, Jian Bai1, Minjuan Xu4, Lei Feng5, Qingying Zhang1*, Wenhan Lin1*   

  1. 1. State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China
    2. The First Institute of Oceanography, State Oceanic Administration (SOA), Qingdao 266061, China
    3. Qingdao University of Science & Technology, Qingdao 266042, China
    4. Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, China
    5. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2014-05-12 Revised:2014-05-27 Online:2014-08-31 Published:2014-06-10
  • Contact: Tel./Fax: 86-10-82806188
  • Supported by:
    Grants from COMRA (Grant No. DY125-15-T-01) and SOA (Grant No. 2010319123366025-4), National High Technology Development Project (863 Project, Grant No. 2011AA10A202-2), National Key Technologies R&D Program (Grant No. 2011BAE06B04).


Chromatographic separation of themarine-derived bacterium Bacillus licheniformis resulted in the isolation of two new cyclic lipopeptides named ai-C16 surfactin (1) and ai-C14 surfactin (2), together with iso-C15 surfactin and iso-C16 surfactin. The structures of the new cyclic lipopeptides were determined through extensive spectroscopic analysis. The sequences ofthe amino acids in cyclic nucleus were established by the ESI-MS/MS fragmentation, which provided an efficient method to detect lipopeptides from bacterium extracts without separation.

Key words: Marine-derived bacterium, Bacillus licheniformis, Cyclic lipopeptides, ai-C16 surfactin, ai-C14 surfactin, UPLC-ESI-MS/MS fragmentation

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