Journal of Chinese Pharmaceutical Sciences ›› 2016, Vol. 25 ›› Issue (5): 373-379.DOI: 10.5246/jcps.2016.05.042

• Original articles • Previous Articles     Next Articles

Optimization of ultrasound assisted extraction of six major compounds from Cimicifugae Rhizome

Wei Chen1, Qin Yuan1, Junping Cheng1, Huinan Ge1, Guorong Jiang1, Rong Chen2*   

  1. 1. Suzhou Hospital of Traditional Chinese Medicine; Suzhou Academy of Wumen Chinese Medicine, Suzhou 215009, China
    2. Suzhou Institute for Food and Drug Control, Suzhou 215104, China
  • Received:2015-11-23 Revised:2016-01-25 Online:2016-05-30 Published:2016-02-26
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Cimicifugae Rhizome (RC) is one of many commonly used traditional Chinese medicines. This study used Box-Behnken Design (BBD), a widely used form of response surface methodology (RSM), to optimize the conditions of the ultrasound-assisted extraction (UAE) for the extraction of phenolic compounds from CimicifugaeRhizome. The influence of three independent variables,including ethanol concentration (%), extraction time (min) and solvent-to-material ratio (mL/g), on the extraction yields of phenolic compounds were examined. The results demonstrated that the optimal ultrasound-assisted extraction (UAE) conditions were as follows: ethanol concentration of 68%, an extraction time of 19 min, and solvent-to-material ratio of 30 mL/g for the extraction of caffeic acid, cimicifugoside, ferulic acid, isoferulicacid, cimifugin, 4'-O-β-glucopyranosyl-5-O-methylvisamminol. These results demonstrated the suitability of UAE for the extraction of phenolic compounds from CimicifugaeRhizome.

Key words: Ultrasound assisted extraction, Response surface methodology, CimicifugaeRhizome, HPLC

CLC Number: 

Supporting: Suzhou Youth science and technology plan (Grant No. KJXW2015028).