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Journal of Chinese Pharmaceutical Sciences ›› 2020, Vol. 29 ›› Issue (11): 804-812.DOI: 10.5246/jcps.2020.11.073

• Original articles • Previous Articles     Next Articles

Determination and pharmacokinetic study of p-hydroxyphenethyl anisate following intravenous and oral administration in rats by RP-HPLC method

Xiuwei Yang*, Youbo Zhang, Wei Xu   

  1. State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2020-08-23 Revised:2020-09-18 Online:2020-11-30 Published:2020-10-07
  • Contact: Tel.: +86-10-82801569, E-mail: xwyang@bjmu.edu.cn
  • About author:Dr. Xiuwei Yang got his doctorate in Toyama Medical and Pharmaceutical University of Japan in 1992. He worked as a postdoctoral research fellow at School of Pharmaceutical Sciences from Beijing Medical University in 1992–1994. In 1994.9, he joined Peking University School of Pharmaceutical Sciences and worked as a professor. His major research interests are the absorption, distribution, metabolism, excretion, toxicity and activity of drugs (ADMET/Act.), complex system exploration of traditional Chinese Medicine, biological transformation of natural drug and/or Chinese medicinal chemical compositions, innovative drug discovery and natural medicinal chemistry.
  • Supported by:
    The National Natural Science Foundation of China (Grant No. 30672609), National High Technology Research and Development Program of China (Grant No. 2002AA2Z343C, 2004AA2Z3783), National Sciences and Technology Program of China (Grant No. 2006BAI06A01-02), and Beijing Municipal Special-Purpose Science Foundation of China (Grant No. Z0004105040311).

Abstract:

In the present study, we developed a rapid and specific reversed-phase high-performance liquid chromatographic (RP-HPLC) method for the quantification of p-hydroxyphenethyl anisate (HPA), one of the main bioactive constituents of the roots and rhizomes ofNotopterygium incisumand N. franchetii, in rat plasma after an intravenous (20 mg/kg, i.v.) and an intragastrical (200 mg/kg,i.g.) administration to rats, respectively. The method involved a plasma clear-up step using liquid-liquid extraction by EtOAc, followed by RP-HPLC separation and detection. Separation of HPAwas performed on an analytical DiamonsilTM ODS C18 column with the mobile phase of MeOH–H2O at ratios of 75:25 (v/v) for i.v. and 70:30 (v/v) for i.g. administration. The flow-rate was 1.0 mL/min, and UV detection was performed at 256 nm. The calibration curves were linear over the ranges of 0.05–5.0 μg/mL (r2 = 0.9984) for i.v. and 0.5–10.0 μg/mL (r2 = 0.9995) for i.g. administrationin rat plasma. The extraction recoveries were in the range of 82.01%–87.97%. The intra- and inter-day precisions were between 1.71% and 3.99%, with accuracies ranging from 91.22% to 110.5%. The absolute bioavailability of an orally administered HPA in rats was about 48.17%. The developed method was suitable for the determination and pharmacokinetic studyof HPA in rat plasma.

 

Key words: p-Hydroxyphenethyl anisate, Pharmacokinetic, Bioavailability, RP-HPLC, Notopterygium

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