Journal of Chinese Pharmaceutical Sciences

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Applications of multiplexed immunohistochemistry/immunofluorescence and multispectral imaging technology in the field of tumor immunotherapy

Wenzhe Li*, Xia Yuan, Bo Xu, Shuxiang Song   

  1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2020-09-05 Revised:2020-09-27 Online:2020-10-31 Published:2020-09-28
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Multiplexed immunohistochemistry/fluorescence (mIHC/IF) in combination with multispectral unmixing is a novelmultitarget histopathological staining and imaging technique. By simultaneously revealing expression level and spatial information for up to eight biomarkers in situ, in addition to a nuclear stain within a single formalin-fixed paraffin-embedded (FFPE) tissue section, this technology can analyze the phenotype, abundance, morphology and intercellular relationship of cells while providing statistically significant results. In recent years, technical improvements have brought new insight into mIHC/IF and multispectral imaging approaches, which have been successfully applied in the field of cancer immunotherapy, specifically in regard to tumor microenvironment research, immunotherapy drug discovery, and prognostic and metastatic risk evaluation. This reviewhighlights the principle, workflow, advantages and disadvantages of the technology, and discusses the latest applications of mIHC/IF-based imaging technology in the field ofTME-related research and immunotherapy drug discovery. 

Key words: Multiplexed immunohistochemistry/immunofluorescence (mIHC/IF), Multispectral imaging, Tumor microenvironment, Tumor immunotherapy

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