Journal of Chinese Pharmaceutical Sciences ›› 2020, Vol. 29 ›› Issue (7): 470-479.DOI: 10.5246/jcps.2020.07.044

• Original articles • Previous Articles     Next Articles

Chemical comparison of Semen Euphorbiae and Semen Euphorbiae Pulveratum by UPLC-Q-TOF/MS coupled with multivariate statistical techniques

王慧楠1, 张景珍1, 崔曰新1, 王思雨1, 高慧1, 张瑶1, 王新杰1, 杨子烨1, 陈梦雨1, 王佩华1, 张桂梅1, 王英姿1*, 张超2*   

  1. 1. 北京中医药大学 中药学院, 北京 102488
    2. 山东中医药大学 药学院, 山东 济南 250355
  • Received:2020-03-28 Revised:2020-04-16 Online:2020-07-31 Published:2020-05-18
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  • Supported by:
    Beijing Natural Science Foundation (Grant No. 7182097), National Natural Science foundation of China (Grant No. 81673597) and National Key Research and Development Program of China (Grant No. 2018YFE0197900).


In the present study, we aimed to assess the chemical composition changes of Semen Euphorbiae (SE) and Semen Euphorbiae Pulveratum (SEP) by UPLC-Q-TOF/MS and multivariate statistical methods. The UPLC-Q-TOF/MS method and SIMCA-P software were used to analyze the changes of chemical components of SE and SEP based on PCA and PLS-DA multivariate statistical methods. A “component-target-disease” network model was constructed by Intelligent Platform for Life Sciences of traditional Chinese medicine (TCM) to predict potential related diseases. The differences of chemical composition were significant between SE and SEP. Under positive ion mode, the amounts of Euphorbia factor L2, L3, L7a, L8, L9 and lathyrol were obviously decreased after processing. Under negative ion mode, the amounts of aesculetin, bisaesculetin, ingenol and cetylic acid were increasedobviously, while Euphorbia factor L1, L4 and L5 were decreased obviously after processing, and the components of euphobiasteroid, aesculetin, lathyrol and linoleic acid among the 14 differentiated compounds were closely related to the SE-related diseases through the “component-target-disease” network model. UPLC-Q-TOF/MS technology in combination with multivariate statisticalmethods had certain advantages in studying the complex changes of chemical composition before and after manufacturing pulveratumof SE. It provided a basis for clarifying the toxicity-attenuated mechanisms of SE manufacturing pulveratum, and laid the foundation for its further development and utilization. 

Key words: Semen Euphorbiae, Semen Euphorbiae Pulveratum, UPLC-Q-TOF/MS, Multivariate statistical techniques, Chemical constituents, Manufacturing pulveratum

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