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Journal of Chinese Pharmaceutical Sciences ›› 2020, Vol. 29 ›› Issue (6): 422-430.DOI: 10.5246/jcps.2020.06.040

• Original articles • Previous Articles     Next Articles

Application of a quantitative 1H NMR method for rapid extraction and determination of the content of paeonol in Cynanchum paniculatum

Xuejiao Li1, Jianwei Dong1*, Xiu Gao2*, Guijun Li1, Junyou Shi1, Yanqing Zhang1   

  1. 1. College of Chemistry and Environmental Science, Qujing Normal University, Qujing 655011, China
    2. College of Biological Resources and Food Engineering, Qujing Normal University, Qujing 655011, China
  • Received:2019-12-14 Revised:2020-03-24 Online:2020-06-30 Published:2020-03-30
  • Contact: Tel.: +86-874-8998658; +86-874-8998620, E-mail: jwdongyn@mail.qjnu.edu.cn; appleartgao@163.com
  • Supported by:
    Science and Technology Project of Yunnan Provincial Department of Science and Technology (Grant No. 2018FD081), Yunnan Local Colleges Applied Basic Research Projects [Grant Nos. 2019FH001(-109), 2017FH001-092, 2017FH001-094 and 2018FH001-019], and Youth Research Foundation of Qujing Normal University in 2019 (Grant No. 2019QN004).

Abstract:

Cynanchum paniculatum (Bunge) Kitagawa is usually used as an herbal medicine for treating many diseases. Paeonol is the main active component, and its content is the key indicator for quality control of C. paniculatum. In the present study, we developed a rapid, accurate and precise method for quantitation of paeonol in C. paniculatum using 1H NMR spectra. The deuteratedsolvent of methanol-d4 enabled satisfactory separation of the signals to be integrated in 1H NMR spectrum. H-6 (δ 7.78) of1H NMR spectrum of C. paniculatum was selected as the feature signal for quantitation, and trimesic acid (TMA) was selected as an internal standard. Validation of the quantitative method was performed in terms of linearity, specificity, repeatability and stability. This is the first time to report quantitative 1H NMR(qHNMR) applied to determine the content of paeonol in C. paniculatum and showed a wider linearity range than the reported quantitation of paeonol in others. The simple extraction of paeonol from C. paniculatum was rapid and will prompt the application of the developed method. This work implied that qHNMR represented a feasible alternative to HPLC-based methods for quantitation of paeonol in C. paniculatum, and it was suitable for the quality control of C. paniculatum. 

Key words: Cynanchum paniculatum, Quantitative 1H NMR, Paeonol, Quantitative determination

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