http://www.jcps.ac.cn

Journal of Chinese Pharmaceutical Sciences ›› 2020, Vol. 29 ›› Issue (5): 355-363.DOI: 10.5246/jcps.2020.05.033

• Original articles • Previous Articles     Next Articles

Research on Moflo XDP high speed cell sorting techniques and parameter optimization

Yufang Sun1, Chen Zheng2, Bo Xu1*   

  1. 1. 北京大学医学部 药学院 天然药物及仿生药物国家重点实验室, 北京 100191 
    2. 贝克曼库尔特商贸(中国)有限公司, 北京 100004
  • Received:2020-02-18 Revised:2020-04-05 Online:2020-05-31 Published:2020-04-12
  • Contact: Tel.: +86-10-82805217, E-mail: xubo@bjmu.edu.cn

Abstract:

This article aims to discuss strategies to operate Moflo XDP high-speed sorting equipment for cell sorting and optimize the instrument to run in the best operating state. Using fluorescent microspheres to quickly calibrate the position of the main liquidflow and the laser beam, adjust the amplitude and frequency according to different size CytoNozzles to find the best breakoff point, and adjust the four-way side flow to achieve the stable maintaining of the equipment, so as to quickly sort sample at high-speed. The absence of air bubbles in the sheath liquid barrel and the liquid path is the key to achieving a stable sorting-maintaining in the sorting process. The accurate calculation of the breakoff point and drop delay is the key to improve sorting efficiency and cell activity.

Key words: Cell sort, Bubble, Maintaining, CV optimization

CLC Number: 

Supporting: