Journal of Chinese Pharmaceutical Sciences ›› 2020, Vol. 29 ›› Issue (4): 252-259.DOI: 10.5246/jcps.2020.04.024

• Original articles • Previous Articles     Next Articles

Identification and discrimination of Ziziphi Spinosae Semen and Ziziphi Mauritianae Semen based on HPLC fingerprint analysis

Hongshuai Yang1, Qiuyi Liu1, Weidong Yin2, Manjiang Xie1, Xiang Ji1, Shuangyu Shao1, Hong Liang1, Pengfei Tu1, Qingying Zhang1*   

  1. 1. State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
    2. Department of Pharmacy, Linzi District People’s Hospital, Zibo 255400, China
  • Received:2019-10-29 Revised:2020-01-03 Online:2020-04-30 Published:2020-01-14
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  • Supported by:
    National Project for Standardization of Chinese Material Medica (Grant No. ZYBZH-Y-JIN-34).


A simple, reproducible and reliable HPLC fingerprint analysis method of Ziziphi Spinosae Semen (ZSS) and Ziziphi Mauritianae Semen (ZMS) was established, and then it was applied to analyze samples collected from different locations. A total of 12 common fingerprint peaks were designated in ZSS and ZMS, respectively, and five of which were definitely identified. Of these common peaks, 10 peaks were presented in both ZSS and ZMS, whereas two were characteristic for ZSS and ZMS, respectively.Furthermore, the two characteristic peaks of ZMS were definitely assigned as 6′′′-sinapoylspinosin and frangufoline by HPLC guided purification and structural elucidation. The HPLC fingerprint analysis was proved to be useful in identifying and discriminating ZSS and ZMS, which was beneficial for quality control of ZSS and its products.

Key words: Ziziphi Spinosae Semen, Ziziphi Mauritianae Semen, Ziziphus jujuba var. spinosa, Ziziphi mauritianae, HPLC fingerprint

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