Journal of Chinese Pharmaceutical Sciences ›› 2020, Vol. 29 ›› Issue (3): 199-205.DOI: 10.5246/jcps.2020.03.017

• Original articles • Previous Articles     Next Articles

Determination of β-amyloid peptides in vitro aggregation process by electrochemistry

Sufang Zhang, Cong Li, Ying Zhang, Juncheng Zou, Yinzhu Cui, Xiaomei Ling*   

  1. Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2019-06-24 Revised:2019-08-15 Online:2020-03-30 Published:2019-12-29
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  • Supported by:
    The National Youth Foundation of China (Grant No. 81803487), National Natural Science Foundation of China (Grant No. 81673392) and Postdoctoral Science Foundation of China (Grant No. 2018M631285).


An impedimetric sensor (EA/mAb/CeZnO/GCE) for detecting β-amyloid peptides (Aβ) was successfully constructed by synthesizing ceria doped zinc oxide (CeZnO) nanoparticles to modify the glassy carbon electrode (GCE) for immobilizing the antibody mAb, and blocking the unbound carboxyl sites with ethanolamine (EA). The sensor exhibited a linear detection range of 10–100 pM and a detection limit of 1.7 pM (S/N = 3), and the relative standard deviations (RSDs) of the inter-electrode and intra-electrodes were less than 3.0%. After it was stored at 4 °C for 2 d, its impedance value was 101.6% of original impedance response. The sensor was used to measure the remaining content of Aβ incubated in vitro. The results showed that after incubation for 48 h and 60 h, the content of aggregated Aβ (oligomers and fibers) almost unchanged, reaching about 90%. This method had the advantages of time-saving, simple procedure, economical, excellent conductivity, good reproducibility and stability.  

Key words: Electrochemical sensor, Electrochemical impedance spectroscopy, β-Amyloid peptides

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