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Journal of Chinese Pharmaceutical Sciences ›› 2019, Vol. 28 ›› Issue (10): 739-748.DOI: 10.5246/jcps.2019.10.070

• Original articles • Previous Articles    

Porcine placental peptides improve neuroblast proliferation and differentiation via enhancement of TrkB and BDNF levels in D-glatacose-induced mouse aging model

Xu Quan1, Caihong Gu3, Feng Yan4, Yaoyao Gao5, Lin Chen6, Rong Wei7, Bingchun Yan1,2*, Rong Hu1,2*   

  1. 1. Department of Pharmacy, Institute of Translational Medicine, Medical College, Yangzhou University,Yangzhou 225001, Jiangsu, China
    2. The Key Laboratory of Syndrome Differentiation and Treatment of Gastric Cancer of the State Administration of Traditional Chinese Medicine, Yangzhou University, Yangzhou 225001, Jiangsu, China
    3. Jiangsu Lianhuan Pharmaceutical Group Co., Ltd., Yangzhou 225009, Jiangsu, China
    4. Jiangsu Yangnong Chemical Group Co., Ltd., Yangzhou 225001, Jiangsu, China
    5. Gaoyou Hospital of Traditional Chinese Medicine, Yangzhou University, Yangzhou 225600, Jiangsu, China
    6. Tongzhou District People's Hospital, Nantong 226300, Jiangsu, China
    7. Nantong Tumor Hospital, Nantong 226361, Jiangsu, China
  • Received:2019-06-30 Revised:2019-08-27 Online:2019-10-31 Published:2019-09-18
  • Contact: Tel.: +86-514-87992215, E-mail: prhurong@163.com; bcyan@yzu.edu.cn
  • Supported by:

    The University Natural Science Research major Project of Jiangsu Province (Grant No. 16KJA310006).

Abstract:

Aging affects the nervous system as well as other organs. In our study, we aimed to study the pharmacological effects and mechanism of porcine placental peptides (PPP) in aging process, and to observe the changes of neuroblast proliferation and differentiation as well as partial gene expression in hippocampus of D-galactose-induced aged mouse. Based on the analysis of experimental results, it was confirmed that PPP significantly improved neurobalst proliferation and differentiation in the mouse hippocampal DG by ki-67 and DCX immunohistochemistry. This result showed that PPP had anti-aging effects on D-galactose-induced aging mouse model. Moreover, we observed up-regulated expressions of BDNF and TrkB proteins and down-regulated expressions of Caspase 3, 8, and 9 proteins in the PPP-treated mouse hippocampus. Therefore, our results showed that PPP obviouslyimproved neuroblast proliferation and differentiation, and its anti-aging effect might berelated to down-regulation of apoptosis-related proteins, including Caspase 3, 8, and 9, via BDNF/TrkB pathway. Our findings provided valuable evidence for its applications in the health and medicine sectors.

Key words: Porcine placenta peptide, Anti-aging, Neuroblast proliferation and differentiation, Apoptosis-related proteins, BDNF/TrkB pathway

CLC Number: 

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