Journal of Chinese Pharmaceutical Sciences ›› 2019, Vol. 28 ›› Issue (8): 537-546.DOI: 10.5246/jcps.2019.08.051

• Original articles • Previous Articles     Next Articles

Simultaneous determination of five phenylethanoid glycosides in Cistanches Herba using quantitative analysis of multi-components by single marker

Ruiyan Li1, Mingbo Zhao2, Pengfei Tu2, Yong Jiang2*   

  1. 1. Department of Pharmacy, Changzhi Medical College, Changzhi 046000, China
    2. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
  • Received:2019-05-18 Revised:2019-06-02 Online:2019-09-02 Published:2019-06-15
  • Contact: Tel.: +86-10-82802719, E-mail:
  • Supported by:

    National Key R & D Program of China (Grant Nos. 2017YFC1702400, 2018YFC1707300 and 2018YFC1707904).


In the present study, a method for the quantitative analysis of multi-components by single marker (QAMS)has been developed and validated for the simultaneous determination of echinacoside (ECH), tubuloside A, acteoside, isoacteoside, and 2'-acetylacteoside inCistanches Herba. ECH was used as the internal standard (IS) to obtain the relative correction factors (RCFs) of the other four phenylethanoid glycosides (PhGs); meanwhile, various influencing factors on RCFs were investigated under differentconditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method (ESM) to verify the feasibility and accuracy of the established QAMS method. No significant differencewas found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM.The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba. 

Key words: Cistanches Herba, Phenylethanoid glycosides, Quantitative analysis of multi-components by single-marker, Relative correction factors

CLC Number: