Journal of Chinese Pharmaceutical Sciences ›› 2019, Vol. 28 ›› Issue (3): 167-173.DOI: 10.5246/jcps.2019.03.016

• Original articles • Previous Articles     Next Articles

Rapid and sensitive HPLC-MS/MS method for quantitative determination of isochlorogenic acid B in rat plasma and its application in pharmacokinetic study

Xin Liu1,2, Bo Zhang1, Dan Mei1, Kai Huang3*   

  1. 1. Department of Pharmacy, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
    2. State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
    3. Drug Clinical Trial Institution, Wuxi People’ Hospital, Nanjing Medical University, Wuxi 214023, China
  • Received:2018-12-30 Revised:2019-01-05 Online:2019-03-30 Published:2019-02-17
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  • Supported by:

    Chinese Academy of Medical Sciences (Grant No. CAMS-2017-I2M-1-011).


Asensitive LC-ESI-MS/MS method for determination of isochlorogenic acid B in rat plasma was developed and validated in the present study. Plasma samples were prepared by a simple protein precipitation with methanol containing resveratrol as internal standard (IS). The chromatographic separation was performed on a Zorbax SB-C18 column (3.5 μm, 2.1 mm×100 mm, Agilent, USA) at a flow rate of 0.2 mL/min using methanol/water containing 0.1% formic acid (v/v) as mobile phase. The detectionwas performed on a triple quadrupole tandem mass spectrometer equipped with Electronic Spray Ion by selected reaction monitoring (SRM) of the transitions at m/z 515.3→352.9 for isochlorogenic acid B and m/z 227.1→143.1 for IS, respectively. The calibration curve of the method was linear over the range of 5–2500 ng/mL (r2 = 0.9982). The intra- and inter-day precisions (R.S.D.%) were less than 12.46%, and the accuracy (R.E.%) was within ±5.80%. Isochlorogenic acid B was sufficiently stable under all relevant analytical conditions. The validated method was successfully applied to the plasma pharmacokinetic studies of isochlorogenic acid B in rats. It was found that isochlorogenic acid B had non-linear pharmacokinetic characteristics in rats within the dosage ranges from 5 to 20 mg/kg. 

Key words: Isochlorogenic acid B, LC-ESI-MS/MS, Plasma concentration, Pharmacokinetics

CLC Number: