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Journal of Chinese Pharmaceutical Sciences ›› 2018, Vol. 27 ›› Issue (12): 855-863.DOI: 10.5246/jcps.2018.12.087

• Original articles • Previous Articles     Next Articles

Chemical constituents and analgesic activity of Aconitum kusnezoffii Reichb.

Qian Li1,2, Shuding Sun1, Meiying Wang2, Caifeng Li2, Dan Yuan1*, Hongzheng Fu2*   

  1. 1. College of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China
    2. State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China
  • Received:2018-06-01 Revised:2018-06-26 Online:2018-12-30 Published:2018-10-08
  • Contact: Tel.: +86-010-82805212, E-mail: drhzfu@sina.com
  • Supported by:

    National Natural Science Foundation of China  (Grant No. 30973628) and the National Science and Technology Major Project of China (Grant No. SQ2018ZX090301).

Abstract:

In the present study,we aimed to investigate the chemical constituents and analgesic activity of Aconitum kusnezoffii Reichb. The isolation and purification of components were achieved by a series of chromatography, including silica gel, Sephadex LH-20 and HPLC. By using spectroscopic analysis, their structures were identified. Using PDE-4A as analgesic target, moleculardocking was conducted between isolated compounds by using Schrodinger software. Neoline is a typical non-ester diterpene alkaloid. It was studied by using the mouse torsion body method and hot plate method. A total of 12 diterpene alkaloids were obtainedand identified as Mesaconitine(1), Bewutine (2), Bewudine (3), Songoramine (4), Songorine (5), Neoline (6), Talasamine (7), isotalatizidine (8), Hokbusine A (9), Mesaconine (10), 8-OEt-14-benzoylmesaconine (11), 8-Methoxy-14-benzoyl-beiwutinine (12).Compounds 9 and 12 were isolated from Aconitum kusnezoffii Reichb. for the first time. Twelve diterpenealkaloids could act on the analgesic target. Neoline is a typical non-ester diterpene alkaloid. It had significant analgesic effect. Diterpene alkaloids were the main components of Aconitum kusnezoffiiReichb., and they had good analgesic activity.

Key words: Aconitum kusnezoffii Reichb., Chemical constituents, Structural identification, Analgesic activity, Molecular docking

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