Journal of Chinese Pharmaceutical Sciences ›› 2018, Vol. 27 ›› Issue (12): 855-863.DOI: 10.5246/jcps.2018.12.087

• Original articles • Previous Articles     Next Articles

Chemical constituents and analgesic activity of Aconitum kusnezoffii Reichb.

Qian Li1,2, Shuding Sun1, Meiying Wang2, Caifeng Li2, Dan Yuan1*, Hongzheng Fu2*   

  1. 1. College of Traditional Chinese Medicine, Shenyang Pharmaceutical University, Shenyang 110016, China
    2. State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing 100191, China
  • Received:2018-06-01 Revised:2018-06-26 Online:2018-12-30 Published:2018-10-08
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  • Supported by:

    National Natural Science Foundation of China  (Grant No. 30973628) and the National Science and Technology Major Project of China (Grant No. SQ2018ZX090301).


In the present study,we aimed to investigate the chemical constituents and analgesic activity of Aconitum kusnezoffii Reichb. The isolation and purification of components were achieved by a series of chromatography, including silica gel, Sephadex LH-20 and HPLC. By using spectroscopic analysis, their structures were identified. Using PDE-4A as analgesic target, moleculardocking was conducted between isolated compounds by using Schrodinger software. Neoline is a typical non-ester diterpene alkaloid. It was studied by using the mouse torsion body method and hot plate method. A total of 12 diterpene alkaloids were obtainedand identified as Mesaconitine(1), Bewutine (2), Bewudine (3), Songoramine (4), Songorine (5), Neoline (6), Talasamine (7), isotalatizidine (8), Hokbusine A (9), Mesaconine (10), 8-OEt-14-benzoylmesaconine (11), 8-Methoxy-14-benzoyl-beiwutinine (12).Compounds 9 and 12 were isolated from Aconitum kusnezoffii Reichb. for the first time. Twelve diterpenealkaloids could act on the analgesic target. Neoline is a typical non-ester diterpene alkaloid. It had significant analgesic effect. Diterpene alkaloids were the main components of Aconitum kusnezoffiiReichb., and they had good analgesic activity.

Key words: Aconitum kusnezoffii Reichb., Chemical constituents, Structural identification, Analgesic activity, Molecular docking

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