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Journal of Chinese Pharmaceutical Sciences ›› 2018, Vol. 27 ›› Issue (5): 342-351.DOI: 10.5246/jcps.2018.05.035

• Original articles • Previous Articles     Next Articles

Genistein-loaded poloxamer 403/407 mixed micelles: preparation and pharmacokinetic study in rats

Yan Cai1, Wei Dai3, Fuhua Qin2*, Jieyin Sun2, Ruilong Wei2   

  1. 1. Department of Pharmacy, Ningbo NO. 9 Hospital, Ningbo 315020, China
    2. Department of Pharmeucital Engineering, Zhejiang Pharmaceutical College, Ningbo 315100, China
    3. Hangzhou Dongxiang Pharmaceutical Technology Co., Ltd., Hangzhou 310051, China
  • Received:2018-01-13 Revised:2018-03-20 Online:2018-05-31 Published:2018-05-11
  • Contact: Tel.: +86-574-88225752, E-mail: qinfh@mail.zjpc.net.cn
  • Supported by:

    The Zhejiang Public Welfare Technology Application Research Project (Grant No. 2015C31100) and the Ningbo Science and Technology Innovation Team Project (Grant No. 2015C110027).

Abstract:

In the present study, we aimed to prepare poloxamer 403/407 mixed micelles in order to improve the solubility and oral bioavailability of genistein.Genistein was incorporated in the mixed poloxamer micelles by thin-film hydration method, and its physicochemical properties, including particle size, zeta potential, entrapment efficiency and drug loading, were investigated.In vitro release of genistein from the mixed micelles was monitored by dialysis method, and pharmacokinetic study of genistein loaded mixed micelles was carried out in rats. We found that the particle size and zeta potential of mixed micelles were (20.31±0.43) nm and (8.94±0.35) mV, with encapsulation efficiency 90.59%±0.67% and drug loading 7.74%±0.05%. Solubility of genistein in mixed micelles reached 3.80 mg/mL, which was about 130 times higher than that in water.Genistein-loaded mixed micelles showed sustained release characteristics in vitro with no burst release phenomenon, but it was faster than suspension.The AUC0t andAUC0of mixed micelles were 196.74% and 204.62% greater than that of genisein suspension, respectively.Consequently,poloxamer 403/407 mixed micelles significantly improved the solubility and oral bioavailability of genistein, which could be used as an effective drug delivery system for oral administration of poorly soluble drugs. 

Key words: Genistein, Poloxamer 407, Poloxamer 403, Micelles, Pharmacokinetics

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