Journal of Chinese Pharmaceutical Sciences ›› 2017, Vol. 26 ›› Issue (11): 805-810.DOI: 10.5246/jcps.2017.11.090

• Original articles • Previous Articles     Next Articles

Simultaneous determination of l-calycanthine, quercetin, and kaempferol in Chimonanthi Nitentis Folium by RP-HPLC

Rengeng Shu1*, Xiluan Yan1,2, Shasha Li1, Pengfei Tu3   

  1. 1. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China
    2. School of Environmental & Chemical Engineering, Nanchang University, Nanchang 330031, China
    3. School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2017-08-03 Revised:2017-09-20 Online:2017-11-30 Published:2017-10-08
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  • Supported by:
    National Natural Science Foundation of China (Grant No. 81360631).


A reversed-phase high-performance liquid chromatography (RP-HPLC) method was established for the simultaneous determination of three major constituents, l-calycanthine, quercetin, and kaempferol, in Chimonanthi Nitentis Folium. The RP-HPLC analysis was carried out using an Agilent HC-C18 (4.6 mm×250 mm, 5 µm) column and a gradient mobile phase consisting of methanol and phosphate buffer (10:90→90:10, v/v) at a flow rate of 1.0 mL/min. The column temperature was 25 ºC and the detection wavelength was set at 239 nm from 0 to 15 min and 365 nm from 16 to 45 min. All calibration curves showed good linearity (R2≥0.9991) within test ranges. Relative standard deviations of repeated analyses were less than 2.64% (n = 6), and the recovery of this method was 97.47%-98.26%. The contents of these three analytes were determined for the samples from different harvest times. The results showed that the l-calycanthine content in herbs increases from 99.94 µg/g to 468.0 µg/g from April to July, whereas the content of quercetin and kaempferol was higher in April and May than that in June and July, which were consistent with the Chinese traditional medicine’s use of Chimonanthi Nitentis Folium in Spring. Herein, a simple, rapid and accurate analytical method is presented and successfully applied for the simultaneous quantitative analysis of alkaloids and flavones from Chimonanthus nitens Oliv.

Key words: Chimonanthus nitens Oliv., HPLC, Flavonoids, Alkaloid, Quality control

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