Journal of Chinese Pharmaceutical Sciences ›› 2017, Vol. 26 ›› Issue (5): 355-359.DOI: 10.5246/jcps.2017.05.037

• Original articles • Previous Articles     Next Articles

DNA methyltransferase inhibitor dramatically alters the secondary metabolism of Pestalotiopsis microspora

Dengfeng Yang1, Fenglou Liu3, Xiaolong Yang2*   

  1. 1. State Key Laboratory of Enzyme Technology, National Engineering Research Centre of Non-food Biorefinery, Guangxi Academy of Sciences, Nanning 530007, China
    2. Innovative Drug Research Centre (IDRC), School of Pharmaceutical Sciences, Chongqing University (Huxi Campus), Chongqing 401331, China
    3. Agricultural College, Ningxia Univerisity, Yinchuan 750021, China

  • Received:2017-03-09 Revised:2017-03-23 Online:2017-05-26 Published:2017-05-09
  • Contact: Tel.: +86-023-65678450, E-mail:
  • Supported by:

    National Natural Science Foundation (Grant No. 31660251), Fundamental Research Funds for the Central Universities (Grant No. 0903005203401), and the Start-up Fund for the “Hundred Young-Talent Scheme” Professorship (Grant No. 0236011104424).


5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isolation of a new compound, 4′-formamidophenyl-5-methoxybenzoate (1), along with seven known polyketides, 4-hydroxybenzoic acid (2), LL-P880α (3), 1′-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one (4), pestalotiollide B (5), pestalotiopyrone G (6), endocrocin (7) and 2′-hydroxy-6′-hydroxymethyl-4′-methylphenyl-2,6-dihydroxy-3-(2-isopentenyl)benzoate (8). HPLC profiles revealed that all compounds except for 4 belonged to the newly induced secondary metabolites. In addition, all compounds were proved to be devoid of significant antifungal activity in the bioassays.

Key words: Epigenetic modifier, Plant endophytic fungi, Pestalotiopsis microspora, Polyketide, Antifungal activity

CLC Number: