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Journal of Chinese Pharmaceutical Sciences

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Simultaneous determination of seven bioactive constituents in Liuwei Wuling tablet by HPLC coupled with DAD

Ling Cheng1,2, Xiaohan Wang1,2, Baode Shen1, Pinghua Xu1, Chengying Sheng1, Juan Zheng1,2, Weibo Liao1, Rong Xu1*, Hailong Yuan1*   

  1. 1. Department of Pharmacy, Air Force General Hospital of PLA, Beijing 100142, China
    2. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
  • Received:2016-11-13 Revised:2017-02-13 Online:2017-04-26 Published:2017-03-08
  • Contact: Tel.: +86-010-66928505, E-mail: yhlpharm@126.com
  • Supported by:

    State Project for Essential Drug Research and Development (Grant No. 2012ZX09J12108-04C) and Special Project of Military Health Ministry (Grant No. 13ZJZ12-5).

Abstract:

We employed high performance liquid chromatography (HPLC) method coupled with diode array detector (DAD) for the determinationof seven major bioactive compounds (forsythoside A, specnuezhenide, phillyrin, schisandrin, schisantherin A, deoxyschizandrin and schisandrin B) in Liuwei Wuling tablet (LWWLT). The HPLC baseline separation was carried out on an Inertsil® ODS-3 column (250 mm×4.6 mm, 5 μm) at 25 °C with a gradient elution system composed of 0.1% phosphoric acid solution and acetonitrile. The detection wavelength was set at 230 nm. Calibration curves for seven compounds showed good linear regressions, with correlation coefficients exceeding 0.999 within the tested concentration range. The limits of detection and quantification of each compound were in the range of 0.03–0.28 μg/mL and 0.11–0.87 μg/mL, respectively. The relative standard deviation values of precision, stability and repeatability were less than 2.34%. The average recovery of all seven constituents ranged from 96.71% to 103.9%. The validated quantification method was successfully applied for the analysis of 10 batches of commercial LWWLT from Shandong Shibo Jindu Pharmaceutical Co., Ltd. These results would make foundations for quality control and the further pharmacology study of LWWLT.

Key words: Simultaneous determination, Seven bioactive constituents, Liuwei Wuling tablet, HPLC-DAD

CLC Number: 

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