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Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (10): 690-694.DOI: 10.5246/jcps.2015.10.089

• Original articles • Previous Articles     Next Articles

Determination of chlorpromazine in porcine muscle using high performance liquid chromatography-tandem mass spectrometry

Lu Zhang, Wen Huang, Xinhui Jiang*   

  1. School of Pharmacy of Chongqing Medical University, Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 400016, China
  • Received:2015-06-16 Revised:2015-07-07 Online:2015-10-21 Published:2015-08-07
  • Contact: Tel./Fax: 86-23-68485161, E-mail: Jiangxinhui3@hotmail.com
  • Supported by:
    Chongqing Natural Science Foundation (Grant No. CSCT2013jcyjA10040).

Abstract:

A rapid and accurate high performance liquid chromatography tandem mass spectrometry (HPLS-MS) method was established for quantification of chlorpromazine in pork. The porcine samples were pretreated with acetonitrile to precipitate proteins and followed by extraction with tert-butyl methyl ether (TBME). The separation was performed on a 5 µm Agilent XDB-C18 column with gradient elution. The mobile phase A was 0.01 mol/Lammonium formate in water and mobile phase B was acetonitrile. The flow rate was at 0.8 mL/min. Quantification was performed on a triple-quadrupole tandem mass spectrometer using the multiple selected reaction monitoring (MRM) mode. Transition of m/z +319.1 to 58.1 was used for the quantification of chlorpromazine. The method was validated at the concentration range of 0.4040 µg/kgto 8.080 µg/kg for chlorpromazine with correlation coefficient of 0.9999. The spiked recoveries were more than 80.0% and the limit of detection (LOD) was 0.052 µg/kg.The developed method, which offers advantages of convenience, rapid, specificity and higher sensitivity, can be used for determination of chlorpromazine hydrochloride in porcine muscles.

Key words: High performance liquid chromatography-tandem mass spectrometry, Chlorpromazine hydrochloride, Porcine muscle, Residual quantification

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