Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (4): 236-240.DOI: 10.5246/jcps.2015.04.031

• Original articles • Previous Articles     Next Articles

Simultaneous determination of seven active components in n-butanol effective fraction of Xiao Chai Hu Tang by HPLC-DAD-ELSD

Hongxia Yuan1,2, Huifeng Li1, Miaorong Pei1*   

  1. 1. Shanxi University of Traditional Chinese Medicine, Taiyuan 030024,China
    2. School of Public Health Science, Shanxi Medical University, Taiyuan 030001, China 
  • Received:2015-01-14 Revised:2015-02-02 Online:2015-04-28 Published:2015-02-22
  • Contact: Tel.: 86-351-2272281
  • Supported by:
    Foundation for Young Scholars of Shanxi Province, China (Grant No. 2011021007-2).


An HPLC-DAD-ELSD method was developed and validated for the simultaneous quantitation of seven active components (liquiritin, baicalin, wogonoside, baicalein, wogonin, ginsenosides Re and ginsenosides Rb1) in n-butanol effective fraction of Xiao Chai Hu Tang. A Diamonsil C18(2) column (4.6 mm × 250 mm, 5 μm) was used as the stationary phase and the mobile phase was consisted of acetonitrile and aqueous phosphate acid (0.05%, v/v). Gradient elution was carried out at the flow rate of 1 mL/min. The detection wavelength was set at 276 nm and an evaporative light scattering detector was also used. Good linearity for all the seven active components was observed. The established method is simple, fast, reliable, and suitable for the quality control of n-butanol effective fraction of Xiao Chai Hu Tang.

Key words: HPLC-DAD-ELSD, n-Butanol effective fraction, Xiao Chai Hu Tang, Simultaneous determination

CLC Number: