Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (4): 231-235.DOI: 10.5246/jcps.2015.04.030

• Original articles • Previous Articles     Next Articles

Analysis of the influence of processing of bran stir-baking on the main components of Paeoniae Radix Alba-Atractylodis Macrocephalae Rhizoma herbal pair by high-performance liquid chromatography

Xinhua Fang1, Xin Wu2, Gang Cao2,3*, Hao Cai3, Baochang Cai2,3*   

  1. 1. TCM Dispensary, Traditional Chinese Medical Hospital of Hangzhou, Hangzhou 310007, China
    2. Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China
    3. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China
  • Received:2015-02-06 Revised:2015-03-11 Online:2015-04-28 Published:2015-03-15
  • Contact: Tel.: 86-571-87195895
  • Supported by:

    The National Natural Science Foundation of China (Grant No. 81202918), the Open Project of National First-Class Key Discipline for Science of Chinese Materia Medica, Nanjing University of Chinese Medicine (Grant No. 2011ZYX2-006), the Science and Technology Project of Hangzhou, China (Grant No. 20130533B68 and 20131813A23), the Chinese Medicine Research Program of Zhejiang Province, China (Grant No. 2014ZQ008 and 2015ZQ011), and the Science Foundation of Zhejiang Chinese Medical University (Grant No. 2011ZY25 and 2013ZZ12).


The crude and processed Paeoniae Radix Alba-Atractylodis Macrocephalae Rhizoma herbal pairs, originated from Bai-zhu-shao-yao-san, are used to treat different diseases clinically. In order to evaluate the crude and processed Paeoniae Radix-Atractylodis MacrocephalaeRhizoma herbal pairs, a simple, easy, and sensitive high-performance liquid chromatography coupled with diode array detectors was developed for simultaneous determination of nine bioactive components in the herbal pairs. The calibration curve exhibited good linearity (r2≥0.9992). The LODs and LOQs were ≤7.30 and 11.53 µg/mL, respectively. The intra-, inter-day and repeatability RSD values of the nine compounds were less than 3.86%, 2.71%, and 4.29%, respectively. The RSD stability values were less than 3.64%. The recovery of the method was in the range of 96.70%–102.10%, with RSD values less than 3.52%. The developed method can be applied to the intrinsic quality control of crude and processed Paeoniae Radix-Atractylodis Macrocephalae Rhizoma herbal pairs.

Key words: High-performance liquid chromatography, Processing, Quantification, Paeoniae Radix Alba, Atractylodis Macrocephalae Rhizoma

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