Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (3): 164-168.DOI: 10.5246/jcps.2015.03.020

• Original articles • Previous Articles     Next Articles

Effect of 5-HMF on protection of hydrogen peroxide-induced injury in human umbilical vein endothelial cells

Yafen Shen1, Xin Wu2, Gang Cao2*, Hao Cai3*   

  1. 1. Zhejiang Provincial Hospital of TCM, Hangzhou 310006, China
    2. Research Center of TCM Processing Technology, Zhejiang Chinese Medical University, Hangzhou 311401, China
    3. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
  • Received:2014-12-12 Revised:2015-01-08 Online:2015-03-29 Published:2015-01-10
  • Contact: Tel.: 0571-87195895
  • Supported by:

    The Chinese Medicine Research Program of Zhejiang Province, China (Grant No. 2014ZQ008); the Science and Technology Project of Hangzhou, China (Grant No. 20130533B68, and 20131813A23), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD, Grant No. 2011ZYX2-006), and the Medical Scientific Research Foundation of Zhejiang Province, China (Grant No. 2013KYB183).


The protective effect of 5-hydroxymethylfurfural (5-HMF) against hydrogen peroxide (H2O2)-induced injured in human umbilical vein endothelial cells (HUVECs) was examined. Treatment of HUVECs with 5-HMF significantly increased their viability, decreased cell apoptosis induced by H2O2, accompanied by the decreased nitric oxide level. Western blotting analysis demonstrated that Bcl-2 expression was increased whereas Caspase-3 and NF-κB expression was decreased in 5-HMF groups. In conclusion, the results showed that 5-HMF could prevent endothelial dysfunction and related complications caused by oxidative stress.

Key words: Processed Fructus Corni, 5-Hydroxymethylfurfural, Nitric oxide, Apoptosis, Hydrogen peroxide

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