Journal of Chinese Pharmaceutical Sciences ›› 2015, Vol. 24 ›› Issue (2): 88-94.DOI: 10.5246/jcps.2015.02.010

• Original articles • Previous Articles     Next Articles

Simultaneous determination of three main analytes of Murraya exotica by HPLC

Bingyu Liu1, Chen Zhang1, Haining Lv1, Pengfei Tu1, Jianyong Xing2, Zhengzhou Han2, Yong Jiang1*   

  1. 1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China
    2. China Resources Sanjiu Medical & Pharmaceutical Co. Ltd., Shenzhen 518110, China
  • Received:2014-12-11 Revised:2014-12-30 Online:2015-02-01 Published:2015-01-01
  • Contact: Tel.: 86-10-82802719
  • Supported by:
    National Natural Science Foundation of China (Grant No. 81222051 and 81473106) and National Key Technology R&D Program “New Drug Innovation” of China (Grant No. 2012ZX09301002-002-002 and 2012ZX09304-005).


Murraya exotica L. is one of the two official source species of traditional Chinese medicine Murrayae Folium et Cacumen. At present, a rapid HPLC analysis method to simultaneously determine two major coumarins, hainanmurpanin (1) and meranzin (2), and one main flavonoid,3,5,6,7,3′,4′,5′-heptamethoxyflavone (3), from the leaves and twigs of M. exotica was established. The analysis was performed on a DIKMA Spursil C18 column (4.6 mm×250 mm, 5 µm) at a flow rate of 1 mL/min, using acetonitrileH2O (5:5, v/v) as mobile phase. The column temperature was 25 ºC, and the detected wavelength was at 320 nm. The three analytes (13) were separated well with good linearity, precision, stability and repeatability. The average recoveries were in the range of 100.52%101.97%, with RSD less than 1.73%. Twenty batches of M. exotica from different habitats were detected, and the sum of the contents of 13 was in the range of 1.557.45 mg/g, respectively. Besides, the contents of these three analytes were also determined for the samples from different medicinal parts (stems, lateral branches, mixture of twigs and leaves) and different harvest times. The results showed that the contents of these three analytes in leaves and twigs are much higher than those in the stems or lateral branches; the plants harvested from June or October contain more active compoundsthan those from the other months. The above results proved that this high performance and simple HPLC assay can be readily utilized as a practical method for the quality control of M. exotica.

Key words: Murraya exotica L., Coumarin, Flavonoid, Quantification, HPLC

CLC Number: