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Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (10): 674-680.DOI: 10.5246/jcps.2014.10.086

• Original articles • Previous Articles     Next Articles

Hemocompatibility and cytocompatibility of diblock copolymer poly(2-ethyl-2-oxazoline)-poly(D,L-lactide)-based micelles

Shujin Ma1, Yanfang Li2, Yong Zhao1, Yanxia Zhou1, Jinwen Li1, Yajie Gao1, Yushu Li1, Xinru Li1*, Yan Liu1*, Xinglin Wang3   

  1. 1. Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University Health Sciences Center, Beijing 100191, China
    2. Central Lab, Peking University Third Hospital, Beijing 100191, China
    3. State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China
  • Received:2014-04-11 Revised:2014-05-15 Online:2014-10-31 Published:2014-05-20
  • Contact: Tel.: 86-10-82801508
  • Supported by:

    National Natural Science Foundation of China (Grant No. 81172990), the National Key Science Research Program of China (Grant No. 973 Program, 2009CB930300), Innovation Team of Ministry of Education (Grant No. BMU20110263) and the Open Project Program of State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research.

Abstract:

A synthetic diblock copolymerpoly(2-ethyl-2-oxazoline)-poly(D,L-lactide) (PEOz-PLA) can self-assemble into micelles with an increased efficiency of drug delivery. However, the interactions of blood-micelles and cell-micelles remain unclear. In the present study, we aimed to assess the hemocompatibility and cytocompatibility of PEOz-PLA micelles in order to clarify its potentials as carriers for drug delivery. Blood compatibility of the micelles was evaluated by hemolysis analysis, coagulation test, platelet activation investigation and assessment of their interaction with protein. The results revealed that PEOz-PLA micelles had a favorable blood compatibility. In addition, PEOz-PLA micelles showed a good cytocompatibility through SRB assay, presenting only negligible cytotoxicity when incubated with KBv cells. Taken together, PEOz-PLA micelles could be used as a hemocompatible andcytocompatible drug carrier for intravenous administration.

Key words: Hemocompatibility, Cytocompatibility, PEOz-PLA micelles, Hemolysis, Blood clotting, Platelet activation, Protein adsorption

CLC Number: 

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