Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (9): 649-653.DOI: 10.5246/jcps.2014.09.083

• Original articles • Previous Articles     Next Articles

A validated HPLC method for the determination of cefazolin sodium level in rabbit synovial fluid

Jing Li1, Weiwei Lin1, Dai Li2, Yingfang Ao2*, Haisong Yang1, Xiaomei Ling1*   

  1. 1. Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
    2. Institute of Sports Medicine, The Third Hospital of Peking University, Beijing 100191, China
  • Received:2014-04-06 Revised:2014-05-04 Online:2014-09-23 Published:2014-05-14
  • Contact: Tel.: 86-10-82802414, 86-10-82267390
  • Supported by:
    National Natural Science Foundation (Grant No. 81373372) and Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20110001110021 and 20130001110059).


A sensitive and specific high performance liquid chromatography method was established for measuring cefazolin sodium level in rabbit synovial fluid.Acetonitrile was used to precipitate proteins and antipyrine was used as internal standard. Samples were analyzed on a Dionex Ultimate U3000 HPLC system equipped with Phenomenex Luna C18 column (150 mm×4.60 mm, 5 μm, 100 A). The mobile phase consisted of acetonitrile and 0.1% formic acid in water. The flow rate was 1 mL/min. The detectionwavelength was set at 272 nm. The column temperature was maintained at 25 ºC.The calibration curve was linear in the range of 1.0-100.0 μg/mL(r2 = 0.9999). The limit of detection (LOD, S/N = 3) was 0.07 μg/mL. The limit of quantitation (LOD, S/N = 10) was 0.22 μg/mL. The recovery of cefazolin sodium (low, medium and high) was 124.6%, 117.8%, and 100.6% (RSD% = 1.9%, 4.0%, 1.1%, n = 5), respectively. The intra-day and inter-day precision values were in the range of 0.5%-2.7%.The method was simple, sensitive and reliable. It can be used for the quantitative determination of cefazolin sodium in rabbit synovial fluid.

Key words: Cefazolin sodium, Rabbit synovial fluid, HPLC

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