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Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (8): 533-542.DOI: 10.5246/jcps.2014.08.069

• Original articles • Previous Articles     Next Articles

Detection and quantification of cyclo-dopa amides in Portulaca oleracea L. by HPLC-DAD and HPLC-ESI-MS/MS

Zezhao Jiao, Haina Wang, Peipei Wang, Hongxiang Sun, Su Yue, Lan Xiang*   

  1. School of Pharmaceutical Sciences, Shandong University, Shandong Jinan 250012, China
  • Received:2014-04-08 Revised:2014-04-21 Online:2014-08-31 Published:2014-05-10
  • Contact: Tel.: 86-531-88382028, Fax: 86-531-88382548
  • Supported by:
    National Natural Science Foundation of China (Grant No. 81073005), Shandong Province Young and Middle-Aged Scientists Research Awards Fund (Grant No. BS2010YY032), Scientific Research Foundation for Returned Overseas Scholars, Ministry of Education of China (Grant No. 42) and Innovation Project of Shandong University (Grant No. 2012TS102).

Abstract:

In the present study, we aimed to detect and quantify cyclo-dopa amides in Portulaca oleracea L. Together with four known ones (oleracein A–D), we further identified eight new cyclo-dopa amides, named oleracein H–O, using an HPLC-DAD combined with an improved HPLC-ESI-MS/MS method. A standardized HPLC fingerprint of cyclo-dopa amides was generated for the first time through analyzing 11 batches of P. oleracea. Simultaneously, the contents of two major amides, oleracein A (OA) and oleracein B (OB), and the total amides (TID) were determined. Our results showed that the contents of OA and OB of P. oleracea ranged from 35.00 to 151.93 mg/kg and from 40.00 to 150.44 mg/kg, respectively. Moreover, TID ranged from 314.16 to 928.60 mg/kg (calculated by OA), or from 475.83 to 1393.00 mg/kg (calculated by OB). Taken together, our newly developed method could be used for the quality assessment and quality control of this herb.

Key words: Portulaca oleracea L., Cyclo-dopa amide, Detection and quantification

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