Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (6): 378-384.DOI: 10.5246/jcps.2014.06.051

• Original articles • Previous Articles     Next Articles

Effects of (–)-5-hydroxy-equol on the lifespan and stress resistance of Caenorhabditis elegans

Conghui Zhang1, Xiuling Wang1*, Xiaolin Liang1,2, Honglei Zhang1, Qinghong Hao1   

  1. 1. College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China
    2. North China Pharmaceutical Group Corporation, Shijiazhuang 050000, China
  • Received:2014-03-24 Revised:2014-03-31 Online:2014-06-28 Published:2014-04-20
  • Contact: Tel.: 86-312-7528257, Fax: 86-312-7528265
  • Supported by:
    National Natural Science Foundation of China (Grant No. 31170058) and the Service Center for Experts and Scholars of Hebei Province (Grant No. CPRC027).


Caenorhabditis elegans (C. elegans) was used as an animal model to study the effect of (–)-5-hydroxy-equol, a microbial metabolite of isoflavone genistein, on the lifespan, fecundity and resistance against thermal and oxidative stress. The results showed that (–)-5-hydroxy-equol not only significantly increased the lifespan of C. elegans but alsosignificantly enhanced the resistance against thermal and oxidative stress at the concentrations of 0.1 mmol/L and 0.2 mmol/L. However, the fecundity ofC.elegans was not obviously influenced after being exposed to the same concentrations of (–)-5-hydroxy-equol. Furtherstudieson comparative transcriptome analyses and the lifespan of daf-16 (mu86) mutant and daf-2 (e1370) mutantindicated that(–)-5-hydroxy-equol prolonged the lifespan of C.elegansthrough DAF-2/DAF-16 Insulin/IGF-1 signaling pathway.This is the first report that (–)-5-hydroxy-equol was able to increase the lifespan and improve the thermal and oxidative stress tolerance of C. elegans.

Key words: (–)-5-Hydroxy-equol, Caenorhabditis elegans, Lifespan, Thermal stress, Oxidative stress, Fecundity, Insulin/IGF-1 signaling pathwa

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