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Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (6): 378-384.DOI: 10.5246/jcps.2014.06.051

• Original articles • Previous Articles     Next Articles

Effects of (–)-5-hydroxy-equol on the lifespan and stress resistance of Caenorhabditis elegans

Conghui Zhang1, Xiuling Wang1*, Xiaolin Liang1,2, Honglei Zhang1, Qinghong Hao1   

  1. 1. College of Life Sciences, Agricultural University of Hebei, Baoding 071000, China
    2. North China Pharmaceutical Group Corporation, Shijiazhuang 050000, China
  • Received:2014-03-24 Revised:2014-03-31 Online:2014-06-28 Published:2014-04-20
  • Contact: Tel.: 86-312-7528257, Fax: 86-312-7528265
  • Supported by:
    National Natural Science Foundation of China (Grant No. 31170058) and the Service Center for Experts and Scholars of Hebei Province (Grant No. CPRC027).

Abstract:

Caenorhabditis elegans (C. elegans) was used as an animal model to study the effect of (–)-5-hydroxy-equol, a microbial metabolite of isoflavone genistein, on the lifespan, fecundity and resistance against thermal and oxidative stress. The results showed that (–)-5-hydroxy-equol not only significantly increased the lifespan of C. elegans but alsosignificantly enhanced the resistance against thermal and oxidative stress at the concentrations of 0.1 mmol/L and 0.2 mmol/L. However, the fecundity ofC.elegans was not obviously influenced after being exposed to the same concentrations of (–)-5-hydroxy-equol. Furtherstudieson comparative transcriptome analyses and the lifespan of daf-16 (mu86) mutant and daf-2 (e1370) mutantindicated that(–)-5-hydroxy-equol prolonged the lifespan of C.elegansthrough DAF-2/DAF-16 Insulin/IGF-1 signaling pathway.This is the first report that (–)-5-hydroxy-equol was able to increase the lifespan and improve the thermal and oxidative stress tolerance of C. elegans.

Key words: (–)-5-Hydroxy-equol, Caenorhabditis elegans, Lifespan, Thermal stress, Oxidative stress, Fecundity, Insulin/IGF-1 signaling pathwa

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