Journal of Chinese Pharmaceutical Sciences ›› 2014, Vol. 23 ›› Issue (5): 335-337.DOI: 10.5246/jcps.2014.05.047

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Application of recombinant enzymes in the production of human insulin

Chunsheng Leng*, Hang Cui, Shunan Li   

  1. Tonghua Dongbao Pharmaceutical Co. Ltd., Jilin 134001, China
  • Received:2014-01-06 Revised:2014-01-22 Online:2014-05-23 Published:2014-02-23
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In the present study, the cleavage activities of both recombinant and bovine enzymes(trypsin and carboxy peptidase B) were determined using proinsulin fusion protein. The proteolysis products were analyzed with HPLC.We found that recombinant enzymes had stronger activities and produced fewer by-products. In addition, recombinant enzymes were more efficient in the production of human insulin than bovine enzymes.

Key words: Recombinant trypsin, Human insulin, Recombinant carboxy peptidase B, Recombinant enzymes, Recombinant human insulin

CLC Number: 

Supporting: null