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Simultaneous determination of seven alkaloids and two flavonoid glycosides in Wuzhuyu decoction by RP-HPLC-DAD

Chuan-Qin Hu, Xiu-Wei Yang*   

  1. State Key Laboratory of Natural and Biomimetic Drugs; Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2012-02-29 Revised:2012-05-08 Online:2012-06-25 Published:2012-06-25
  • Contact: Xiu-Wei Yang*

Abstract:

A reversed-phase high-performance liquid chromatography (RP-HPLC) method with diode array detector was established to simultaneously determine nine bioactive compounds in Wuzhuyu decoction, namely dehydroevodiamine, isorhamnetin-7-O-rutinoside, 10-hydroxyrutaecarpine, diosmetin-7-O-β-D-glucopyranoside, evodiamine, rutaecarpine, 1-methyl-2-n-nonyl-4(1H)quinolone, evocarpine, and dihydroevocarpine. The RP-HPLC assay was performed on a reversed-phase C18 column with a gradient elution. The mobile phases consisted of methanol and water containing 1% (v/v) acetic acid. The flow rate was 1.0 mL/min and the detection wavelength was set at 300 nm. All calibration curves showed good linearity (r2>0.9941) within test ranges. The results of intra-day precision, inter-day precision, and accuracy were satisfactory. The overall recoveries were in the range of 90.13%-102.48%. The method was successfully applied to quantify nine bioactive compounds in six samples. Baseline resolution for all nine major compounds was achieved. This method could be used as a suitable quality control assay for Wuzhuyu decoction.

Key words: High-performance liquid chromatography, Wuzhuyu decoction, Alkaloids, Flavonoid glycosides

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