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Quantification of eugenol and bancroftione in Caryophylli Fructus using high-performance liquid chromatography

Shu-Juan Liu, Zong-Tao Lin, Hong Wang, Shi-Zhong Chen*   

  1. Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100191, China
  • Received:2010-06-09 Revised:2010-10-15 Online:2010-11-30 Published:2010-11-30
  • Contact: Shi-Zhong Chen*


For the first time, we have utilized high-performance liquid chromatography (HPLC) to simultaneously quantify the eugenol and bancroftione in Caryophylli Fructus. The optimized parameters included: Inertsil ODS-4 column (150 mm×4.6 mm, 5 µm); column temperature: 35 ºC; mobile phase: methanol-water (65:35, v/v); flow rate: 1.0 mL/min; detection wavelength: 280 nm. Eugenol and bancroftione showed good linear relationships with peak areas within the range of (0.0998-0.8982) mg/mL (r = 0.9999) and (0.1474-1.3266) mg/mL (r = 0.9999), respectively. The average recoveries were 102.52% and 100.96% for eugenol and bancroftione, respectively. Our results showed that the established method is simple, rapid, and accurate with good reproducibility to evaluate the quality of Caryophylli Fructus.

Key words: Caryophylli Fructus, Eugenol, Bancroftione, Quantification, HPLC

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